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Maeda Y, Soda M, Ito K, et al. Efficient production of active TNF-alpha by albumin signal peptide. Biochem Mol Biol Int. 1997;42(4):825-32doi: 10.1080/15216549700203261.
Maeda, Y., Soda, M., Ito, K., & Sato, K. (1997). Efficient production of active TNF-alpha by albumin signal peptide. Biochemistry and molecular biology international, 42(4), 825-32. https://doi.org/10.1080/15216549700203261
Maeda, Y, et al. "Efficient production of active TNF-alpha by albumin signal peptide." Biochemistry and molecular biology international vol. 42,4 (1997): 825-32. doi: https://doi.org/10.1080/15216549700203261
Maeda Y, Soda M, Ito K, Sato K. Efficient production of active TNF-alpha by albumin signal peptide. Biochem Mol Biol Int. 1997 Jul;42(4):825-32. doi: 10.1080/15216549700203261. PMID: 19856300.
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Biochem Mol Biol Int. 1997 Jul;42(4):825-32. doi: 10.1080/15216549700203261.

Efficient production of active TNF-alpha by albumin signal peptide.

Biochemistry and molecular biology international

Y Maeda, M Soda, K Ito, K Sato

Affiliations

  1. Department of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Japan.

PMID: 19856300 DOI: 10.1080/15216549700203261

Abstract

TNF-alpha is initially synthesized as a membrane-anchored precursor protein and processed proteolytically by a matrix metalloproteinase (MMP)-like enzyme. In order to establish an efficient expression system of TNF-alpha in mammalian cells without involvement of the extracellular enzyme, an expression plasmid (pCN-alb-TNF) was constructed with a signal sequence of the rat albumin gene as a module for secretion. The highest level of production of TNF-alpha was observed in the clone CT-3 by SDS-PAGE and Western blot analysis. Biological activity of the secretion was revealed by repression of catalase gene expression in hepatoma cells and cytotoxity to L929 cells. Attachment of the signal peptide to mature form resulted in the enhancement of production of the cytokine.

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