Zhongguo Zhong Yao Za Zhi. 2010 Feb;35(3):272-4. doi: 10.4268/cjcmm20100303.

[Determination of paprazine in Polygonum orientale of different commodities by HPLC].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

[Article in Chinese]
Jie Song, Yanjun Zhai, Rongxiang Wang, Na Li

PMID: 20422986 DOI: 10.4268/cjcmm20100303

Abstract

OBJECTIVE: To establish a method for the content determination of paprazine in Polygonum orientale, and provide a new evaluation index for the quality control for P. orientale.

METHOD: HPLC was carried out on Agilent C18 (4.6 mm x 150 mm, 5 microm) with methanol-water (40:60) as mobile phase. The flow rate was 1.0 mL x min(-1) and the wavelength of UV detector was set at 290 nm.

RESULT: The calibration curve was linear in the range of 0. 038-0.190 microg (r = 0.9999). The average recovery was 98.7% with the RSD of 2.36%.

CONCLUSION: The method appears to be simple, accurate and reproducible, it can be used to determine the content of paprazine in P. orientale.

Substances

• Drugs, Chinese Herbal

MeSH terms

• Chromatography, High Pressure Liquid / methods
• Drugs, Chinese Herbal / analysis
• Drugs, Chinese Herbal / chemistry
• Polygonum / chemistry

Publication Types

• English Abstract
• Journal Article