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Williams RG, Dimaline R, Varro A, et al. Cholecystokinin octapeptide in rat central nervous system: Immunocytochemical studies using a monoclonal antibody that does not react with CGRP. Neurochem Int. 1987;11(4):433-42doi: 10.1016/0197-0186(87)90033-7.
Williams, R. G., Dimaline, R., Varro, A., Isetta, A. M., Trizio, D., & Dockray, G. J. (1987). Cholecystokinin octapeptide in rat central nervous system: Immunocytochemical studies using a monoclonal antibody that does not react with CGRP. Neurochemistry international, 11(4), 433-42. https://doi.org/10.1016/0197-0186(87)90033-7
Williams, R G, et al. "Cholecystokinin octapeptide in rat central nervous system: Immunocytochemical studies using a monoclonal antibody that does not react with CGRP." Neurochemistry international vol. 11,4 (1987): 433-42. doi: https://doi.org/10.1016/0197-0186(87)90033-7
Williams RG, Dimaline R, Varro A, Isetta AM, Trizio D, Dockray GJ. Cholecystokinin octapeptide in rat central nervous system: Immunocytochemical studies using a monoclonal antibody that does not react with CGRP. Neurochem Int. 1987;11(4):433-42. doi: 10.1016/0197-0186(87)90033-7. PMID: 20501191.
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Neurochem Int. 1987;11(4):433-42. doi: 10.1016/0197-0186(87)90033-7.

Cholecystokinin octapeptide in rat central nervous system: Immunocytochemical studies using a monoclonal antibody that does not react with CGRP.

Neurochemistry international

R G Williams, R Dimaline, A Varro, A M Isetta, D Trizio, G J Dockray

Affiliations

  1. Department of Physiology and Pharmacology, Medical and Biological Sciences Building, University of Southampton, Southampton, SO9 3TU, U.K.; MRC Secretory Control Group, Physiological Laboratory, University of Liverpool, U.K.

PMID: 20501191 DOI: 10.1016/0197-0186(87)90033-7

Abstract

The biological activity of the important neuropeptide cholecystokinin octapeptide (CCK8) resides at the C-terminus. Antibodies with C-terminal specificity have been reported to cross-react with a different neuropeptide, calcitonin gene related peptide (CGRP) and this has frustrated the interpretation of immunohistochemical studies. We describe here the properties of a monoclonal antibody to the CCK-related peptide, caerulein, that reacts with the C-terminal region of CCK8, but does not react with CGRP in radioimmunoassay or immunohistochemistry. The distribution of CCK-like activity revealed by immunohistochemistry using this antibody broadly resembles that described previously with a single major exception: in the dorsal horn of the spinal cord. The results support the suggestion that apparent CCK activity in the terminals of rat primary sensory neurones is due to cross-reactivity with CGRP.

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