Display options
Cite
Holmes S, Dockray GJ. Identification by radioimmunoassay and HPLC of morphine modulatory peptide-immunoreactivity in rat spinal cord and brain. Neurochem Int. 1989;14(4):477-82doi: 10.1016/0197-0186(89)90039-9.
Holmes, S., & Dockray, G. J. (1989). Identification by radioimmunoassay and HPLC of morphine modulatory peptide-immunoreactivity in rat spinal cord and brain. Neurochemistry international, 14(4), 477-82. https://doi.org/10.1016/0197-0186(89)90039-9
Holmes, S, and Dockray, G J. "Identification by radioimmunoassay and HPLC of morphine modulatory peptide-immunoreactivity in rat spinal cord and brain." Neurochemistry international vol. 14,4 (1989): 477-82. doi: https://doi.org/10.1016/0197-0186(89)90039-9
Holmes S, Dockray GJ. Identification by radioimmunoassay and HPLC of morphine modulatory peptide-immunoreactivity in rat spinal cord and brain. Neurochem Int. 1989;14(4):477-82. doi: 10.1016/0197-0186(89)90039-9. PMID: 20504453.
Copy Download .nbib Format: NLM
Share it on

Neurochem Int. 1989;14(4):477-82. doi: 10.1016/0197-0186(89)90039-9.

Identification by radioimmunoassay and HPLC of morphine modulatory peptide-immunoreactivity in rat spinal cord and brain.

Neurochemistry international

S Holmes, G J Dockray

Affiliations

  1. MRC Secretory Control Group, Physiological Laboratory, University of Liverpool, England.

PMID: 20504453 DOI: 10.1016/0197-0186(89)90039-9

Abstract

Two so-called morphine modulatory peptides, an octapeptide and an octadecapeptide, have recently been isolated from bovine spinal cord. We have raised antibodies to the octapeptide (Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH(2): FF-8), which in radioimmunoassay react with peptides terminating in Arg-Phe-NH(2). This dipeptide is common to both the morphine modulatory peptides and the molluscan neuropeptide FMRF amide. The distribution and molecular forms of immunoreactive peptides were examined in the rat central nervous system and gastrointestinal tract. Highest concentrations of FF-8-like immunoreactivity were found in the dorsal spinal cord, brain stem and hypothalamus. The immunoreactive material in central nervous system extracts was resolved by reversed phase HPLC into three peaks of activity, the two largest peaks eluted in similar positions to the standard octapeptide and octadecapeptide. It appears that previously observed FMRF amide-like immunoreactivity in the rat central nervous system corresponds to peptides immunochemically and chromatographically similar to the two bovine spinal cord peptides.

Publication Types