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Trump BF, Berezesky IK, Elliget KA, et al. Nephrotoxicity in vitro: Role of ion deregulation in signal transduction following injury-Studies utilizing digital imaging fluorescence microscopy. Toxicol In Vitro. 1990;4(4):409-14doi: 10.1016/0887-2333(90)90091-7.
Trump, B. F., Berezesky, I. K., Elliget, K. A., Smith, M. A., & Phelps, P. C. (1990). Nephrotoxicity in vitro: Role of ion deregulation in signal transduction following injury-Studies utilizing digital imaging fluorescence microscopy. Toxicology in vitro : an international journal published in association with BIBRA, 4(4), 409-14. https://doi.org/10.1016/0887-2333(90)90091-7
Trump, B F, et al. "Nephrotoxicity in vitro: Role of ion deregulation in signal transduction following injury-Studies utilizing digital imaging fluorescence microscopy." Toxicology in vitro : an international journal published in association with BIBRA vol. 4,4 (1990): 409-14. doi: https://doi.org/10.1016/0887-2333(90)90091-7
Trump BF, Berezesky IK, Elliget KA, Smith MA, Phelps PC. Nephrotoxicity in vitro: Role of ion deregulation in signal transduction following injury-Studies utilizing digital imaging fluorescence microscopy. Toxicol In Vitro. 1990;4(4):409-14. doi: 10.1016/0887-2333(90)90091-7. PMID: 20702205.
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Toxicol In Vitro. 1990;4(4):409-14. doi: 10.1016/0887-2333(90)90091-7.

Nephrotoxicity in vitro: Role of ion deregulation in signal transduction following injury-Studies utilizing digital imaging fluorescence microscopy.

Toxicology in vitro : an international journal published in association with BIBRA

B F Trump, I K Berezesky, K A Elliget, M A Smith, P C Phelps

Affiliations

  1. Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Maryland Institute for Emergency Medical Services Systems, Baltimore, MD 21201, USA.

PMID: 20702205 DOI: 10.1016/0887-2333(90)90091-7

Abstract

Over the years, many approaches have been utilized for studying in vitro toxicity in the kidney. These have included the use of isolated perfused kidneys, renal slices, isolated nephron explants and cultured tubular epithelium. Currently, in vitro systems of either primary cultures or cell lines make it possible to use newly developed fluorescent probes and digital imaging fluorescence microscopy coupled with image analysis to quantify various ions (e.g. [Ca(2+)](i), [Mg(2+)](i), [Na(+)](i) and [H(+)](i)) in individual live cells. Methods have been developed for the primary culture of rat, rabbit and human proximal tubular epithelium and these cultures are being utilized for the study of acute cell injury and for the comparison of animal cell data with that of human cells. In the current studies, the fluorescent probe, Fura 2, was used to observe changes in [Ca(2+)](i) as they relate to cell injury. The results show that changes in [Ca(2+)](i) begin very early after treatments with a variety of agents that produce lethal and sublethal toxic injuries. These injuries can increase [Ca(2+)](i) from its normal 100 nm level to a 2 mum level. The rise in [Ca(2+)](i) precedes early bleb formation and cell death. Elevated [Ca(2+)](i) may also activate enzymes such as lipases and proteases that lead to cell death.

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