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Redstone HA, Buie WD, Hart DA, et al. The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing. Gastroenterol Res Pract. 2010;2010doi: 10.1155/2010/672453.
Redstone, H. A., Buie, W. D., Hart, D. A., Wallace, L., Hornby, P. J., Sague, S., Holst, J. J., & Sigalet, D. L. (2010). The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing. Gastroenterology research and practice, 2010. https://doi.org/10.1155/2010/672453
Redstone, Heather A, et al. "The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing." Gastroenterology research and practice vol. 2010 (2010). doi: https://doi.org/10.1155/2010/672453
Redstone HA, Buie WD, Hart DA, Wallace L, Hornby PJ, Sague S, Holst JJ, Sigalet DL. The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing. Gastroenterol Res Pract. 2010;2010. doi: 10.1155/2010/672453. Epub 2010 Oct 04. PMID: 20953406; PMCID: PMC2952794.
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Gastroenterol Res Pract. 2010;2010. doi: 10.1155/2010/672453. Epub 2010 Oct 04.

The effect of glucagon-like Peptide-2 receptor agonists on colonic anastomotic wound healing.

Gastroenterology research and practice

Heather A Redstone, William D Buie, David A Hart, Laurie Wallace, Pamela J Hornby, Sarah Sague, Jen J Holst, David L Sigalet

Affiliations

  1. Division of Colorectal Surgery, Department of Surgery, Faculty of Medicine, University of Calgary, Calgary, AB, Canada T2N 1N4.

PMID: 20953406 PMCID: PMC2952794 DOI: 10.1155/2010/672453

Abstract

Background. Glucagon-like peptide 2 (GLP-2) is an intestinal specific trophic hormone, with therapeutic potential; the effects on intestinal healing are unknown. We used a rat model of colonic healing, under normoxic, and stress (hypoxic) conditions to examine the effect of GLP-2 on intestinal healing. Methods. Following colonic transection and reanastomosis, animals were randomized to one of six groups (n = 8/group): controls, native GLP-2, long-acting GLP-2 (GLP-2- MIMETIBODY, GLP-2-MMB), animals were housed under normoxic or hypoxic (11%  O(2)) conditions. Animals were studied five days post-operation for anastomotic strength and wound characteristics. Results. Anastomotic bursting pressure was unchanged by GLP-2 or GLP-2-MMB in normoxic or hypoxic animals; both treatments increased crypt cell proliferation. Wound IL-1β increased with GLP-2; IFNγ with GLP-2 and GLP-2-MMB. IL-10 and TGF-β were decreased; Type I collagen mRNA expression increased in hypoxic animals while Type III collagen was reduced with both GLP-2 agonists. GLP-2 MMB, but not native GLP-2 increased TIMP 1-3 mRNA levels in hypoxia. Conclusions. The effects on CCP, cytokines and wound healing were similar for both GLP-2 agonists under normoxic and hypoxic conditions; anastomotic strength was not affected. This suggests that GLP-2 (or agonists) could be safely used peri-operatively; direct studies will be required.

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