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Wang LM, Wen JX, Yuan JL, et al. Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development. Cytotechnology. 2011;64(2):165-72doi: 10.1007/s10616-011-9402-5.
Wang, L. M., Wen, J. X., Yuan, J. L., Cang, M., & Liu, D. J. (2012). Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development. Cytotechnology, 64(2), 165-72. https://doi.org/10.1007/s10616-011-9402-5
Wang, L M, et al. "Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development." Cytotechnology vol. 64,2 (2012): 165-72. doi: https://doi.org/10.1007/s10616-011-9402-5
Wang LM, Wen JX, Yuan JL, Cang M, Liu DJ. Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development. Cytotechnology. 2012 Mar;64(2):165-72. doi: 10.1007/s10616-011-9402-5. Epub 2011 Oct 22. PMID: 22021024; PMCID: PMC3279582.
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Cytotechnology. 2012 Mar;64(2):165-72. doi: 10.1007/s10616-011-9402-5. Epub 2011 Oct 22.

Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development.

Cytotechnology

L M Wang, J X Wen, J L Yuan, Ming Cang, D J Liu

Affiliations

  1. Key Laboratory of China Education Ministry for Research of Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, 010021, People's Republic of China.

PMID: 22021024 PMCID: PMC3279582 DOI: 10.1007/s10616-011-9402-5

Abstract

This study aimed to assess the efficiency and effects of insulin-like growth factor receptor-1 (IGF-IR) siRNA knockdown during bovine preimplantation embryonic development. In oocytes injected with IGF-IR siRNA, the relative IGF-IR mRNA levels compared to controls were 28% and 46% at 6 and 24 h after injection, respectively. With respect to the injection of IGF-IR siRNA in zygotes, 24 h after injection the relative levels of IGF-IR mRNA and protein in the two-cell embryos were 74% and 78% of those in the controls, respectively. IGF-IR siRNA reduced blastocyst formation (23.2%) compared to siRNA controls (33.0%) and uninjected oocytes (35.4%; P < 0.05) and the number of viable cells per IGF-IR siRNA-treated blastocyst (64 ± 3) was significantly reduced, compared to control siRNA and uninjected blastocysts (81 ± 3 and 116 ± 4; P < 0.01). In conclusion, IGF-IR siRNA knockdown reduces the development of bovine embryos, and microinjection in zygotes can decrease blastocyst cell number.

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