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Gulliksen A, Keegan H, Martin C, et al. Towards a "Sample-In, Answer-Out" Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System. J Oncol. 2011;2012:905024doi: 10.1155/2012/905024.
Gulliksen, A., Keegan, H., Martin, C., O'Leary, J., Solli, L. A., Falang, I. M., Grønn, P., Karlgård, A., Mielnik, M. M., Johansen, I. R., Tofteberg, T. R., Baier, T., Gransee, R., Drese, K., Hansen-Hagge, T., Riegger, L., Koltay, P., Zengerle, R., Karlsen, F., Ausen, D., & Furuberg, L. (2012). Towards a "Sample-In, Answer-Out" Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System. Journal of oncology, 2012905024. https://doi.org/10.1155/2012/905024
Gulliksen, Anja, et al. "Towards a "Sample-In, Answer-Out" Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System." Journal of oncology vol. 2012 (2012): 905024. doi: https://doi.org/10.1155/2012/905024
Gulliksen A, Keegan H, Martin C, O'Leary J, Solli LA, Falang IM, Grønn P, Karlgård A, Mielnik MM, Johansen IR, Tofteberg TR, Baier T, Gransee R, Drese K, Hansen-Hagge T, Riegger L, Koltay P, Zengerle R, Karlsen F, Ausen D, Furuberg L. Towards a "Sample-In, Answer-Out" Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System. J Oncol. 2012;2012:905024. doi: 10.1155/2012/905024. Epub 2011 Dec 22. PMID: 22235204; PMCID: PMC3253481.
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J Oncol. 2012;2012:905024. doi: 10.1155/2012/905024. Epub 2011 Dec 22.

Towards a "Sample-In, Answer-Out" Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System.

Journal of oncology

Anja Gulliksen, Helen Keegan, Cara Martin, John O'Leary, Lars A Solli, Inger Marie Falang, Petter Grønn, Aina Karlgård, Michal M Mielnik, Ib-Rune Johansen, Terje R Tofteberg, Tobias Baier, Rainer Gransee, Klaus Drese, Thomas Hansen-Hagge, Lutz Riegger, Peter Koltay, Roland Zengerle, Frank Karlsen, Dag Ausen, Liv Furuberg

Affiliations

  1. NorChip AS, Industriveien 8, 3490 Klokkarstua, Norway.

PMID: 22235204 PMCID: PMC3253481 DOI: 10.1155/2012/905024

Abstract

The paper presents the development of a "proof-of-principle" hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (n = 28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%-85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a "sample-in, answer-out" diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.

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