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Yi Y, Wang Z, Li M, et al. Preparation and purification of monoclonal antibodies against chloramphenicol. Cytotechnology. 2011;64(2):157-63doi: 10.1007/s10616-011-9401-6.
Yi, Y., Wang, Z., Li, M., Zhu, K., & Ying, G. (2012). Preparation and purification of monoclonal antibodies against chloramphenicol. Cytotechnology, 64(2), 157-63. https://doi.org/10.1007/s10616-011-9401-6
Yi, Yu, et al. "Preparation and purification of monoclonal antibodies against chloramphenicol." Cytotechnology vol. 64,2 (2012): 157-63. doi: https://doi.org/10.1007/s10616-011-9401-6
Yi Y, Wang Z, Li M, Zhu K, Ying G. Preparation and purification of monoclonal antibodies against chloramphenicol. Cytotechnology. 2012 Mar;64(2):157-63. doi: 10.1007/s10616-011-9401-6. Epub 2011 Dec 11. PMID: 22160396; PMCID: PMC3279585.
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Cytotechnology. 2012 Mar;64(2):157-63. doi: 10.1007/s10616-011-9401-6. Epub 2011 Dec 11.

Preparation and purification of monoclonal antibodies against chloramphenicol.

Cytotechnology

Yu Yi, Zhuhuan Wang, Min Li, Keyin Zhu, Guoqing Ying

Affiliations

  1. College of Pharmaceutical Science, Zhejiang University of Technology, Chaowang road 18, Hangzhou, 310014, China.

PMID: 22160396 PMCID: PMC3279585 DOI: 10.1007/s10616-011-9401-6

Abstract

Monoclonal antibodies (McAbs) against chloramphenicol (CAP) were produced to detect CAP residues, which could be toxic and possesses a potential threat to human health. The CAP-BSA conjugate was obtained by bovine serum albumin (BSA) coupled with CAP, and used to immunize the mice. The splenocytes from the immunized mice were fused with mouse myeloma cells SP2/0 to form hybridoma, which may secrete McAbs against CAP. Hybridomas 1D(1) and 3G(12) secreting McAbs against CAP were obtained by screening. Ascites containing McAbs were prepared by injecting 1 x 10(6) cells of hybridoma 1D(1) and 3G(12) into the abdomen of mice. Protein A affinity chromatography was used to purify McAbs against CAP in a single chromatographic step with recovery yield above 80% and purity above 95% and full recovery of antibody activity. Experiments showed that McAb 3G(12) was highly specific for CAP and had no cross-reactivity with analogues which have a structure similar to CAP. The IC(50) value was 50.8 ng/mL.

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