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Raimondi E, Scariolo S, Vagnarelli P, et al. Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing. Cytotechnology. 1987;1(1):13-7doi: 10.1007/BF00351116.
Raimondi, E., Scariolo, S., Vagnarelli, P., De Sario, A., & De Carli, L. (1987). Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing. Cytotechnology, 1(1), 13-7. https://doi.org/10.1007/BF00351116
Raimondi, E, et al. "Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing." Cytotechnology vol. 1,1 (1987): 13-7. doi: https://doi.org/10.1007/BF00351116
Raimondi E, Scariolo S, Vagnarelli P, De Sario A, De Carli L. Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing. Cytotechnology. 1987 Oct;1(1):13-7. doi: 10.1007/BF00351116. PMID: 22358434.
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Cytotechnology. 1987 Oct;1(1):13-7. doi: 10.1007/BF00351116.

Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing.

Cytotechnology

E Raimondi, S Scariolo, P Vagnarelli, A De Sario, L De Carli

Affiliations

  1. Dipartimento di Genetica e Microbiologia 'A. Buzzati-Traverso', Univ. di Pavia, Via S. Epifanio 14, 27100, Pavia, Italy.

PMID: 22358434 DOI: 10.1007/BF00351116

Abstract

Individual chromosomes can be identified by means of in situ hybridization with DNA probes for chromosome-specific repetitive sequences. The efficiency and sensitivity of the method are strictly dependent on the characteristics of the probes and the experimental conditions. Using three probes with different copy numbers, we demonstrated that the target chromosomes can be visualized in interphase when the homologous sequences are repeated at least 50 times.Possible applications of interphase analysis to clinical cytogenetics and mutagenicity testing are discussed.

References

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