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Tachibana H, Kim JY, Shirahata S. Building high affinity human antibodies by altering the glycosylation on the light chain variable region in N-acetylglucosamine-supplemented hybridoma cultures. Cytotechnology. 1997;23(1):151-9doi: 10.1023/A:1007980032042.
Tachibana, H., Kim, J. Y., & Shirahata, S. (1997). Building high affinity human antibodies by altering the glycosylation on the light chain variable region in N-acetylglucosamine-supplemented hybridoma cultures. Cytotechnology, 23(1), 151-9. https://doi.org/10.1023/A:1007980032042
Tachibana, H, et al. "Building high affinity human antibodies by altering the glycosylation on the light chain variable region in N-acetylglucosamine-supplemented hybridoma cultures." Cytotechnology vol. 23,1 (1997): 151-9. doi: https://doi.org/10.1023/A:1007980032042
Tachibana H, Kim JY, Shirahata S. Building high affinity human antibodies by altering the glycosylation on the light chain variable region in N-acetylglucosamine-supplemented hybridoma cultures. Cytotechnology. 1997 Jan;23(1):151-9. doi: 10.1023/A:1007980032042. PMID: 22358531; PMCID: PMC3449873.
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Cytotechnology. 1997 Jan;23(1):151-9. doi: 10.1023/A:1007980032042.

Building high affinity human antibodies by altering the glycosylation on the light chain variable region in N-acetylglucosamine-supplemented hybridoma cultures.

Cytotechnology

H Tachibana, J Y Kim, S Shirahata

Affiliations

  1. Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, 812, Japan.

PMID: 22358531 PMCID: PMC3449873 DOI: 10.1023/A:1007980032042

Abstract

We attempted to improve antibody affinity by varying glycosylation on the light chain variable region. The human hybridoma line HB4C5 produces an antibody reactive to lung adenocarcinoma, which possess a N-glycosylated carbohydrate chain on the light chain hypervariable region. It has been shown that altering this carbohydrate structure can be accomplished by varying the level of N-acetylglucosamine in glucose free medium, a change in the carbohydrate chain could be induced which resulted in modifying antigen binding. By culturing the cells in media containing more than 20 mM N-acetylglucosamine, cells produced antibody with 10 fold improved affinity as compared with antibody produced in 20 mM glucose-containing medium. A newly induced light chain glycoform produced in the N-acetylglucosamine-containing medium was shown to be responsible for this antigen binding enhancement. Addition of glucose in the N-acetylglucosamine-containing media led to decreased antibody affinity and slightly inhibited production of a new light chain in a dose-dependent manner. Combination of 20 mM N-acetylglucosamine and 0.5 mM glucose gave a higher antibody production without the decrease of the antigen binding. These results indicate that optimization of N-glycosylation on the light chain, which leads to higher antigen binding, can be accomplished by adjusting a ratio of glucose and N-acetylglucosamine in the culture medium.

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