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Vitale M, Rizzoli R, Mariani AR, et al. Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry. Cytotechnology. 1989;2(1):59-62doi: 10.1007/BF00365415.
Vitale, M., Rizzoli, R., Mariani, A. R., Neri, L. M., Facchini, A., & Papa, S. (1989). Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry. Cytotechnology, 2(1), 59-62. https://doi.org/10.1007/BF00365415
Vitale, M, et al. "Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry." Cytotechnology vol. 2,1 (1989): 59-62. doi: https://doi.org/10.1007/BF00365415
Vitale M, Rizzoli R, Mariani AR, Neri LM, Facchini A, Papa S. Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry. Cytotechnology. 1989 Feb;2(1):59-62. doi: 10.1007/BF00365415. PMID: 22358581.
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Cytotechnology. 1989 Feb;2(1):59-62. doi: 10.1007/BF00365415.

Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry.

Cytotechnology

M Vitale, R Rizzoli, A R Mariani, L M Neri, A Facchini, S Papa

Affiliations

  1. Istituto di Anatomia Umana Normale, Bologna, Italy.

PMID: 22358581 DOI: 10.1007/BF00365415

Abstract

A flow cytometric methodology was set up to assess the binding capability of peripheral blood NK and T cells to the K562 tumor cell line. Differential side scatter characteristics between effectors and targets were used to analyze conjugated and unconjugated cells. The previous labeling of NK and T cells with anti-Leu 11c and anti-Leu 4 monoclonal antibodies, allowed the distinction between unconjugated non-fluorescent and conjugated fluorescent targets and the percentual evaluation of bound anti-Leu 11c(+) and anti-Leu 4(+) cells.

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