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Hunt SM, Pak SC, Bridges MW, et al. Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium. Serum-free growth of IGF-I-producing CHO cells. Cytotechnology. 1997;24(1):55-64doi: 10.1023/A:1007969502256.
Hunt, S. M., Pak, S. C., Bridges, M. W., Gray, P. P., & Sleigh, M. J. (1997). Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium. Serum-free growth of IGF-I-producing CHO cells. Cytotechnology, 24(1), 55-64. https://doi.org/10.1023/A:1007969502256
Hunt, S M, et al. "Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium. Serum-free growth of IGF-I-producing CHO cells." Cytotechnology vol. 24,1 (1997): 55-64. doi: https://doi.org/10.1023/A:1007969502256
Hunt SM, Pak SC, Bridges MW, Gray PP, Sleigh MJ. Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium. Serum-free growth of IGF-I-producing CHO cells. Cytotechnology. 1997 May;24(1):55-64. doi: 10.1023/A:1007969502256. PMID: 22358597; PMCID: PMC3449609.
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Cytotechnology. 1997 May;24(1):55-64. doi: 10.1023/A:1007969502256.

Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium. Serum-free growth of IGF-I-producing CHO cells.

Cytotechnology

S M Hunt, S C Pak, M W Bridges, P P Gray, M J Sleigh

Affiliations

  1. Department of Biotechnology, University of New South Wales, Kensington, NSW, Australia.

PMID: 22358597 PMCID: PMC3449609 DOI: 10.1023/A:1007969502256

Abstract

Insulin-like growth factor I has similar mitogenic effects to insulin, a growth factor required by most cells in culture, and it can replace insulin in serum-free formulations for some cells. Chinese Hamster Ovary cells grow well in serum-free medium with insulin and transferrin as the only exogenous growth factors. An alternative approach to addition of exogenous growth factors to serum-free medium is transfection of host cells with growth factor-encoding genes, permitting autocrine growth. Taking this approach, we constructed an IGF-I heterologous gene driven by the cytomegalovirus promoter, introduced it into Chinese Hamster Ovary cells and examined the growth characteristics of Insulin-like growth factor I-expressing clonal cells in the absence of the exogenous factor. The transfected cells secreted up to 500 ng/10(6) cells/day of mature Insulin-like growth factor I into the conditioned medium and as a result they grew autonomously in serum-free medium containing transferrin as the only added growth factor. This growth-stimulating effect, observed under both small and large scale culture conditions, was maximal since no further improvement was observed in the presence of exogenous insulin.

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