Display options
Cite
Castro PM, Ison AP, Hayter PM, et al. CHO cell growth and recombinant interferon-γ production: Effects of BSA, Pluronic and lipids. Cytotechnology. 1995;19(1):27-36doi: 10.1007/BF00749752.
Castro, P. M., Ison, A. P., Hayter, P. M., & Bull, A. T. (1995). CHO cell growth and recombinant interferon-γ production: Effects of BSA, Pluronic and lipids. Cytotechnology, 19(1), 27-36. https://doi.org/10.1007/BF00749752
Castro, P M, et al. "CHO cell growth and recombinant interferon-γ production: Effects of BSA, Pluronic and lipids." Cytotechnology vol. 19,1 (1995): 27-36. doi: https://doi.org/10.1007/BF00749752
Castro PM, Ison AP, Hayter PM, Bull AT. CHO cell growth and recombinant interferon-γ production: Effects of BSA, Pluronic and lipids. Cytotechnology. 1995 Jan;19(1):27-36. doi: 10.1007/BF00749752. PMID: 22358902.
Copy Download .nbib Format: NLM
Share it on

Cytotechnology. 1995 Jan;19(1):27-36. doi: 10.1007/BF00749752.

CHO cell growth and recombinant interferon-γ production: Effects of BSA, Pluronic and lipids.

Cytotechnology

P M Castro, A P Ison, P M Hayter, A T Bull

Affiliations

  1. Research School of Biosciences, Biological Laboratory, University of Kent, CT2 7NJ, Canterbury, UK.

PMID: 22358902 DOI: 10.1007/BF00749752

Abstract

The role of bovine serum albumin in mammalian cell cultures and the possibility of its substitution by other components in a serum-free medium has been investigated. In this study, BSA was shown to be important for growth and product formation in CHO cells expressing recombinant human interferon-γ. There were indications that its stimulating growth effect was dependent on the source of BSA used and probably was related to the purification procedure used for the production of the desired albumin fraction. Cell growth did not occur in the absence of BSA but at low concentration (1 mg ml(-1)) it was stimulated by the addition of a combination of a commercial lipid mixture plus Pluronic F68. However, under the latter conditions IFN-γ production was adversely effected. The importance of individual lipid components was investigated using a statistical approach based on a Plackett-Burman design. Linoleic acid was identified as a positive variable for cell growth while cholesterol was identified as a negative variable for both cell growth and IFN-γ production. When a combination of linoleic acid plus Pluronic F68 was included in the formulation of low BSA medium, cell growth was similar to that at high BSA concentration (5 mg ml(-1)) but the IFN-γ concentration was significantly reduced (ca. 45%).

References

  1. Biotechnol Appl Biochem. 1995 Feb;21(1):87-100 - PubMed
  2. Cytotechnology. 1991;5 Suppl 2:S35-51 - PubMed
  3. Biotechnol Bioeng. 1992 Nov;40(9):1045-55 - PubMed
  4. J Biotechnol. 1995 Apr 15;39(2):165-73 - PubMed
  5. Cytotechnology. 1988 Nov;1(4):319-29 - PubMed
  6. Biotechnol Bioeng. 1993 Nov 5;42(9):1077-85 - PubMed
  7. Appl Microbiol Biotechnol. 1991 Feb;34(5):559-64 - PubMed
  8. Cytotechnology. 1991 Jul;6(3):233-40 - PubMed
  9. Methods Enzymol. 1986;121:277-92 - PubMed
  10. Appl Microbiol Biotechnol. 1992 Oct;38(1):84-90 - PubMed
  11. Anal Biochem. 1980 Mar 1;102(2):255-70 - PubMed
  12. Trends Biotechnol. 1991 Sep;9(9):316-24 - PubMed
  13. J Cell Physiol. 1983 Apr;115(1):31-6 - PubMed
  14. Biotechnol Bioeng. 1988 Oct 5;32(8):1015-28 - PubMed
  15. Appl Microbiol Biotechnol. 1993 Jan;38(4):520-5 - PubMed
  16. Biotechnology (N Y). 1995 Apr;13(4):389-92 - PubMed
  17. Biotechnol Prog. 1993 May-Jun;9(3):298-308 - PubMed
  18. Cytotechnology. 1994;15(1-3):209-15 - PubMed
  19. In Vitro Cell Dev Biol. 1990 Aug;26(8):769-78 - PubMed
  20. Biotechnol Bioeng. 1993 Apr 15;41(9):868-77 - PubMed
  21. J Immunol Methods. 1991 May 17;139(1):135-44 - PubMed
  22. Biotechnol Bioeng. 1990 Nov;36(9):911-20 - PubMed
  23. Cytotechnology. 1991 Jan;5(1):15-30 - PubMed
  24. J Exp Med. 1987 Jun 1;165(6):1761-6 - PubMed
  25. Biochem Pharmacol. 1988 Feb 15;37(4):569-71 - PubMed
  26. Exp Cell Res. 1980 Mar;126(1):121-6 - PubMed
  27. J Biotechnol. 1991 Feb;17(2):155-67 - PubMed
  28. J Biotechnol. 1992 Sep;25(3):289-306 - PubMed

Publication Types