Display options
Cite
Pepperkok R, Schneider C, Philipson L, et al. Single cell assay with an automated capillary microinjection system. Cytotechnology. 1991;5:93-8doi: 10.1007/BF00736821.
Pepperkok, R., Schneider, C., Philipson, L., & Ansorgel, W. (1991). Single cell assay with an automated capillary microinjection system. Cytotechnology, 593-8. https://doi.org/10.1007/BF00736821
Pepperkok, R, et al. "Single cell assay with an automated capillary microinjection system." Cytotechnology vol. 5 (1991): 93-8. doi: https://doi.org/10.1007/BF00736821
Pepperkok R, Schneider C, Philipson L, Ansorgel W. Single cell assay with an automated capillary microinjection system. Cytotechnology. 1991 Feb;5:93-8. doi: 10.1007/BF00736821. PMID: 22358967.
Copy Download .nbib Format: NLM
Share it on

Cytotechnology. 1991 Feb;5:93-8. doi: 10.1007/BF00736821.

Single cell assay with an automated capillary microinjection system.

Cytotechnology

R Pepperkok, C Schneider, L Philipson, W Ansorgel

Affiliations

  1. European Molecular Biology Laboratory, Heidelberg, Germany.

PMID: 22358967 DOI: 10.1007/BF00736821

Abstract

An automated capillary microinjection system with computer controlled positioning of the cells and of the capillary, and its applications and advantages arc described. About 1500 injections are possible in one hour, with high reproducibility. In cytoplasmic and nuclear injections more than 90% and 85% of the cells are successfully injected. Using FITC-Dextran at a concentration of 0.5% as a fluorescently labelled coinjection marker, 99% of the cells can be retrieved in cultrue medium even 48 hours after unjection. The coordinates of the cells are stored in the computer and accuracy in statistical evaluation of experiments is improved in coparison to the manual techniques. Methods for preparatin and handling of glass capillaries were developed resulting in reproducible form and significantly reduced clogging rate. The improved characteristics offered by this system are demonstrated in studies leading to the confirmation of existence of mRNA(s) inhibiting cell proliferation. Functional screening by cell injections of cDNA libraries and of size fractionated mRNA molecules can be performed efficiently with the automated microinjection system.

References

  1. Exp Cell Res. 1988 Oct;178(2):369-76 - PubMed
  2. Exp Cell Res. 1982 Jul;140(1):31-7 - PubMed
  3. Nature. 1990 Jun 28;345(6278):813-5 - PubMed
  4. J Virol. 1988 Sep;62(9):3341-7 - PubMed
  5. J Biochem Biophys Methods. 1988 Aug;16(4):283-92 - PubMed
  6. Biochem J. 1984 Oct 15;223(2):281-91 - PubMed
  7. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6748-52 - PubMed

Publication Types