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Lanciotti M, Montaldo PG, Cornaglia-Ferraris P, et al. Morphological and biochemical modifications during neuroblastoma cell differentiation. Cytotechnology. 1991;5:178-9doi: 10.1007/BF00736843.
Lanciotti, M., Montaldo, P. G., Cornaglia-Ferraris, P., & Ponzoni, M. (1991). Morphological and biochemical modifications during neuroblastoma cell differentiation. Cytotechnology, 5178-9. https://doi.org/10.1007/BF00736843
Lanciotti, M, et al. "Morphological and biochemical modifications during neuroblastoma cell differentiation." Cytotechnology vol. 5 (1991): 178-9. doi: https://doi.org/10.1007/BF00736843
Lanciotti M, Montaldo PG, Cornaglia-Ferraris P, Ponzoni M. Morphological and biochemical modifications during neuroblastoma cell differentiation. Cytotechnology. 1991 Feb;5:178-9. doi: 10.1007/BF00736843. PMID: 22358989.
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Cytotechnology. 1991 Feb;5:178-9. doi: 10.1007/BF00736843.

Morphological and biochemical modifications during neuroblastoma cell differentiation.

Cytotechnology

M Lanciotti, P G Montaldo, P Cornaglia-Ferraris, M Ponzoni

Affiliations

  1. Pediatric Oncology Research Laboratory, G. Gaslini Children's Hospital, Largo Gerolamo Gaslini 5, 16148, Genova, Italy.

PMID: 22358989 DOI: 10.1007/BF00736843

Abstract

In order to define some distinctive criteria of differentiation, we studied the effects of retinoic acid (RA), γ-interferon (γ-IFN), cytosine arabinoside (ARA-C), nerve growth factor (NGF), tumor necrosis factor (TNF), and 12-0-tetradecanoylphorbol-13 acetate (TPA) on the human neuroblastoma cell line, LAN-5. Intracellular levels of acetylcholinesterase, neuron specific enolase, catecholamines, and related neurotransmitters were evaluated after 8 days of treatment with various inducers. Our data indicate that the most striking biochemical changes accompanying LAN-5 cell differentiation are a shift from a catecholaminergic to a cholinergic phenotype, and/or a drastic increase in the catecholamines levels. Moreover, small changes may be independent of thein vitro maturation. Thus, biochemical phenotyping, more than morphological evaluation, is a fruitful approach to monitor neuronal cell differentiation.

References

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