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Song C, Lang C, Mailliet J, et al. Exploring Chromophore-Binding Pocket: High-Resolution Solid-State H-C Interfacial Correlation NMR Spectra with Windowed PMLG Scheme. Appl Magn Reson. 2011;42(1):79-88doi: 10.1007/s00723-011-0196-6.
Song, C., Lang, C., Mailliet, J., Hughes, J., Gärtner, W., & Matysik, J. (2012). Exploring Chromophore-Binding Pocket: High-Resolution Solid-State H-C Interfacial Correlation NMR Spectra with Windowed PMLG Scheme. Applied magnetic resonance, 42(1), 79-88. https://doi.org/10.1007/s00723-011-0196-6
Song, Chen, et al. "Exploring Chromophore-Binding Pocket: High-Resolution Solid-State H-C Interfacial Correlation NMR Spectra with Windowed PMLG Scheme." Applied magnetic resonance vol. 42,1 (2012): 79-88. doi: https://doi.org/10.1007/s00723-011-0196-6
Song C, Lang C, Mailliet J, Hughes J, Gärtner W, Matysik J. Exploring Chromophore-Binding Pocket: High-Resolution Solid-State H-C Interfacial Correlation NMR Spectra with Windowed PMLG Scheme. Appl Magn Reson. 2012 Feb;42(1):79-88. doi: 10.1007/s00723-011-0196-6. Epub 2011 Feb 11. PMID: 22303079; PMCID: PMC3260431.
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Appl Magn Reson. 2012 Feb;42(1):79-88. doi: 10.1007/s00723-011-0196-6. Epub 2011 Feb 11.

Exploring Chromophore-Binding Pocket: High-Resolution Solid-State H-C Interfacial Correlation NMR Spectra with Windowed PMLG Scheme.

Applied magnetic resonance

Chen Song, Christina Lang, Jo Mailliet, Jon Hughes, Wolfgang Gärtner, Jörg Matysik

PMID: 22303079 PMCID: PMC3260431 DOI: 10.1007/s00723-011-0196-6

Abstract

High-resolution two-dimensional (2D) (1)H-(13)C heteronuclear correlation spectra are recorded for selective observation of interfacial 3-5.5 Å contacts of the uniformly (13)C-labeled phycocyanobilin (PCB) chromophore with its unlabeled binding pocket. The experiment is based on a medium- and long-distance heteronuclear correlation (MELODI-HETCOR) method. For improving (1)H spectral resolution, a windowed phase-modulated Lee-Goldburg (wPMLG) decoupling scheme is applied during the t(1) evolution period. Our approach allows for identification of chromophore-protein interactions, in particular for elucidation of the hydrogen-bonding networks and charge distributions within the chromophore-binding pocket. The resulting pulse sequence is tested on the cyanobacterial (Cph1) phytochrome sensory module (residues 1-514, Cph1Δ2) containing uniformly (13)C- and (15)N-labeled PCB chromophore (u-[(13)C,(15)N]-PCB-Cph1Δ2) at 17.6 T.

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