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Martínez H, Buhse T, Rivera M, et al. Effect of the volume-to-surface ratio of cultures on Escherichia coli growth: an experimental and theoretical analysis. Curr Microbiol. 2012;65(1):60-5doi: 10.1007/s00284-012-0128-1.
Martínez, H., Buhse, T., Rivera, M., Ayala, G., Parmananda, P., & Sánchez, J. (2012). Effect of the volume-to-surface ratio of cultures on Escherichia coli growth: an experimental and theoretical analysis. Current microbiology, 65(1), 60-5. https://doi.org/10.1007/s00284-012-0128-1
Martínez, Haydee, et al. "Effect of the volume-to-surface ratio of cultures on Escherichia coli growth: an experimental and theoretical analysis." Current microbiology vol. 65,1 (2012): 60-5. doi: https://doi.org/10.1007/s00284-012-0128-1
Martínez H, Buhse T, Rivera M, Ayala G, Parmananda P, Sánchez J. Effect of the volume-to-surface ratio of cultures on Escherichia coli growth: an experimental and theoretical analysis. Curr Microbiol. 2012 Jul;65(1):60-5. doi: 10.1007/s00284-012-0128-1. Epub 2012 Apr 25. PMID: 22532396.
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Curr Microbiol. 2012 Jul;65(1):60-5. doi: 10.1007/s00284-012-0128-1. Epub 2012 Apr 25.

Effect of the volume-to-surface ratio of cultures on Escherichia coli growth: an experimental and theoretical analysis.

Current microbiology

Haydee Martínez, Thomas Buhse, Marco Rivera, Guadalupe Ayala, P Parmananda, Joaquín Sánchez

Affiliations

  1. Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca, Morelos, Mexico.

PMID: 22532396 DOI: 10.1007/s00284-012-0128-1

Abstract

The growth dynamics of bacterial populations are usually represented by the classical S-shaped profiles composed of lag, exponential and stationary growth phases. Although exceptions to this classical behavior occur, they are normally produced under non-standard conditions such as supply of two carbohydrates as sole carbon source. However, we here report variations in the classic S-shaped growth profiles of Escherichia coli under standard culturing conditions; explicitly, we found growth during transition to the stationary phase wherein the bacterial growth rate inversely depended on the volume-to-surface ratio of cultures (V/S); the reasons for this behavior were experimentally explored. To complement our experimental analysis, a theoretical model that rationalizes the bacterial response was developed; simulations based on the developed model essentially reproduced experimental growth curves. We consequently conclude that the effect of V/S on E. coli growth reflects an interplay between auto-catalytic bacterial growth, bacterial growth auto-inhibition, and, the relief of that inhibition.

References

  1. Science. 1950 Dec 15;112(2920):715-6 - PubMed
  2. Exp Cell Res. 1975 Mar 15;91(2):389-92 - PubMed
  3. Nature. 2007 Oct 18;449(7164):827-34 - PubMed
  4. J Theor Biol. 2007 Jan 21;244(2):326-48 - PubMed
  5. J Bacteriol. 1940 Oct;40(4):547-58 - PubMed
  6. Biochim Biophys Acta. 1966 Mar 28;117(1):22-32 - PubMed
  7. Microb Pathog. 2012 Jul;53(1):49-55 - PubMed
  8. J Bacteriol. 2004 Mar;186(5):1355-61 - PubMed
  9. J Bacteriol. 2009 Jan;191(1):347-54 - PubMed

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