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Frazer JK, Batchelor LA, Bradley DF, et al. Genomic amplification of an endogenous retrovirus in zebrafish T-cell malignancies. Adv Hematol. 2012;2012:627920doi: 10.1155/2012/627920.
Frazer, J. K., Batchelor, L. A., Bradley, D. F., Brown, K. H., Dobrinski, K. P., Lee, C., & Trede, N. S. (2012). Genomic amplification of an endogenous retrovirus in zebrafish T-cell malignancies. Advances in hematology, 2012627920. https://doi.org/10.1155/2012/627920
Frazer, J Kimble, et al. "Genomic amplification of an endogenous retrovirus in zebrafish T-cell malignancies." Advances in hematology vol. 2012 (2012): 627920. doi: https://doi.org/10.1155/2012/627920
Frazer JK, Batchelor LA, Bradley DF, Brown KH, Dobrinski KP, Lee C, Trede NS. Genomic amplification of an endogenous retrovirus in zebrafish T-cell malignancies. Adv Hematol. 2012;2012:627920. doi: 10.1155/2012/627920. Epub 2012 Jun 13. PMID: 22745640; PMCID: PMC3382231.
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Adv Hematol. 2012;2012:627920. doi: 10.1155/2012/627920. Epub 2012 Jun 13.

Genomic amplification of an endogenous retrovirus in zebrafish T-cell malignancies.

Advances in hematology

J Kimble Frazer, Lance A Batchelor, Diana F Bradley, Kim H Brown, Kimberly P Dobrinski, Charles Lee, Nikolaus S Trede

Affiliations

  1. Department of Pediatrics, University of Utah, Salt Lake City, UT 84112, USA.

PMID: 22745640 PMCID: PMC3382231 DOI: 10.1155/2012/627920

Abstract

Genomic instability plays a crucial role in oncogenesis. Somatically acquired mutations can disable some genes and inappropriately activate others. In addition, chromosomal rearrangements can amplify, delete, or even fuse genes, altering their functions and contributing to malignant phenotypes. Using array comparative genomic hybridization (aCGH), a technique to detect numeric variations between different DNA samples, we examined genomes from zebrafish (Danio rerio) T-cell leukemias of three cancer-prone lines. In all malignancies tested, we identified recurring amplifications of a zebrafish endogenous retrovirus. This retrovirus, ZFERV, was first identified due to high expression of proviral transcripts in thymic tissue from larval and adult fish. We confirmed ZFERV amplifications by quantitative PCR analyses of DNA from wild-type fish tissue and normal and malignant D. rerio T cells. We also quantified ZFERV RNA expression and found that normal and neoplastic T cells both produce retrovirally encoded transcripts, but most cancers show dramatically increased transcription. In aggregate, these data imply that ZFERV amplification and transcription may be related to T-cell leukemogenesis. Based on these data and ZFERV's phylogenetic relation to viruses of the murine-leukemia-related virus class of gammaretroviridae, we posit that ZFERV may be oncogenic via an insertional mutagenesis mechanism.

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