Display options
Cite
Li Y, Zhang L, Li M, et al. A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode. Chem Cent J. 2012;6(1):103doi: 10.1186/1752-153X-6-103.
Li, Y., Zhang, L., Li, M., Pan, Z., & Li, D. (2012). A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode. Chemistry Central journal, 6(1), 103. https://doi.org/10.1186/1752-153X-6-103
Li, Yuanting, et al. "A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode." Chemistry Central journal vol. 6,1 (2012): 103. doi: https://doi.org/10.1186/1752-153X-6-103
Li Y, Zhang L, Li M, Pan Z, Li D. A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode. Chem Cent J. 2012 Sep 17;6(1):103. doi: 10.1186/1752-153X-6-103. PMID: 22986118; PMCID: PMC3505160.
Copy Download .nbib Format: NLM
Share it on

Chem Cent J. 2012 Sep 17;6(1):103. doi: 10.1186/1752-153X-6-103.

A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode.

Chemistry Central journal

Yuanting Li, Li Zhang, Meng Li, Zhigang Pan, Dawei Li

Affiliations

  1. Key Laboratory for Advanced Materials & Department of Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P R China. [email protected].

PMID: 22986118 PMCID: PMC3505160 DOI: 10.1186/1752-153X-6-103

Abstract

BACKGROUND: Biosensors have attracted increasing attention as reliable analytical instruments in in situ monitoring of public health and environmental pollution. For enzyme-based biosensors, the stabilization of enzymatic activity on the biological recognition element is of great importance. It is generally acknowledged that an effective immobilization technique is a key step to achieve the construction quality of biosensors.

RESULTS: A novel disposable biosensor was constructed by immobilizing laccase (Lac) with silica spheres on the surface of multi-walled carbon nanotubes (MWCNTs)-doped screen-printed electrode (SPE). Then, it was characterized in morphology and electrochemical properties by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The characterization results indicated that a high loading of Lac and a good electrocatalytic activity could be obtained, attributing to the porous structure, large specific area and good biocompatibility of silica spheres and MWCNTs. Furthermore, the electrochemical sensing properties of the constructed biosensor were investigated by choosing dopamine (DA) as the typical model of phenolic compounds. It was shown that the biosensor displays a good linearity in the range from 1.3 to 85.5 μM with a detection limit of 0.42 μM (S/N = 3), and the Michaelis-Menten constant (Kmapp) was calculated to be 3.78 μM.

CONCLUSION: The immobilization of Lac was successfully achieved with silica spheres to construct a disposable biosensor on the MWCNTs-doped SPE (MWCNTs/SPE). This biosensor could determine DA based on a non-oxidative mechanism in a rapid, selective and sensitive way. Besides, the developed biosensor could retain high enzymatic activity and possess good stability without cross-linking reagents. The proposed immobilization approach and the constructed biosensor offer a great potential for the fabrication of the enzyme-based biosensors and the analysis of phenolic compounds.

References

  1. Anal Chem. 1976 Oct;48(12):1679-86 - PubMed
  2. Chem Cent J. 2007 Nov 29;1:30 - PubMed
  3. Biosens Bioelectron. 2005 Jul 15;21(1):206-11 - PubMed
  4. Analyst. 2011 Apr 7;136(7):1279-87 - PubMed
  5. J Biotechnol. 2011 Oct 20;156(1):39-45 - PubMed
  6. Biosens Bioelectron. 2010 May 15;25(9):2089-94 - PubMed
  7. Anal Bioanal Chem. 2009 Jan;393(2):543-54 - PubMed
  8. Sensors (Basel). 2009;9(1):219-31 - PubMed
  9. Talanta. 2010 Jun 15;81(4-5):1636-42 - PubMed
  10. J Am Chem Soc. 2002 Sep 25;124(38):11242-3 - PubMed
  11. Acc Chem Res. 2011 Nov 15;44(11):1232-43 - PubMed
  12. Biosens Bioelectron. 2006 Jun 15;21(12):2195-201 - PubMed
  13. Biosens Bioelectron. 2010 Feb 15;25(6):1350-5 - PubMed
  14. Biosens Bioelectron. 2009 Mar 15;24(7):2225-31 - PubMed
  15. Biosens Bioelectron. 2011 Mar 15;26(7):3181-6 - PubMed
  16. Chem Cent J. 2011 Dec 02;5:75 - PubMed
  17. Anal Biochem. 2011 Dec 15;419(2):196-204 - PubMed
  18. Trends Analyt Chem. 2008;27(7):619-626 - PubMed

Publication Types