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Adamson J, Hughes S, Azzopardi D, et al. Real-time assessment of cigarette smoke particle deposition in vitro. Chem Cent J. 2012;6(1):98doi: 10.1186/1752-153X-6-98.
Adamson, J., Hughes, S., Azzopardi, D., McAughey, J., & Gaça, M. D. (2012). Real-time assessment of cigarette smoke particle deposition in vitro. Chemistry Central journal, 6(1), 98. https://doi.org/10.1186/1752-153X-6-98
Adamson, Jason, et al. "Real-time assessment of cigarette smoke particle deposition in vitro." Chemistry Central journal vol. 6,1 (2012): 98. doi: https://doi.org/10.1186/1752-153X-6-98
Adamson J, Hughes S, Azzopardi D, McAughey J, Gaça MD. Real-time assessment of cigarette smoke particle deposition in vitro. Chem Cent J. 2012 Sep 10;6(1):98. doi: 10.1186/1752-153X-6-98. PMID: 22958446; PMCID: PMC3443673.
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Chem Cent J. 2012 Sep 10;6(1):98. doi: 10.1186/1752-153X-6-98.

Real-time assessment of cigarette smoke particle deposition in vitro.

Chemistry Central journal

Jason Adamson, Sophie Hughes, David Azzopardi, John McAughey, Marianna D Gaça

Affiliations

  1. British American Tobacco, Group R&D, Regents Park Road, Southampton, SO, 15 8TL, UK. [email protected].

PMID: 22958446 PMCID: PMC3443673 DOI: 10.1186/1752-153X-6-98

Abstract

BACKGROUND: Recently there has been a rapid increase in approaches to assess the effects of cigarette smoke in vitro. Despite a range of gravimetric and chemical methods, there is a requirement to identify simpler and more reliable methods to quantify in vitro whole smoke dose, to support extrapolation and comparisons to human/in vivo dose. We have previously characterised an in vitro exposure system using a Borgwaldt RM20S smoking machine and a chamber exposing cellular cultures to whole smoke at the air-liquid interface. In this study we demonstrate the utility of a quartz crystal microbalance (QCM), using this exposure system, to assess real-time cigarette smoke particulate deposition during a 30 minute smoke exposure. Smoke was generated at various dilutions (1:5-1:400, smoke:air) using two cigarette products, 3R4F Kentucky reference and 1 mg commercially available cigarettes. The QCM, integrated into the chamber, assessed particulate deposition and data generated were compared to traditional chemical spectrofluorometric analysis.

RESULTS: The QCM chamber was able to detect mass differences between the different products within the nanogram range. 3R4F reference cigarette smoke deposition ranged from 25.75 ±2.30 μg/cm2 (1:5) to 0.22 ±0.03 μg/cm2 (1:400). 1 mg cigarette smoke deposition was less and ranged from 1.42 ±0.26 μg/cm2 (1:5), to 0.13 ±0.02 μg/cm2 (1:100). Spectrofluorometric analysis demonstrated statistically significant correlation of particulate deposition with the QCM (p < 0.05), and regression R2 value were 97.4 %. The fitted equation for the linear model which describes the relationship is: QCM = -0.6796 + 0.9744 chemical spectrofluorescence.

CONCLUSIONS: We suggest the QCM is a reliable, effective and simple tool that can be used to quantify smoke particulate deposition in real-time, in vitro and can be used to quantify other aerosols delivered to our chamber for assessment.

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