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Pei C, Xu Y, Jiang JX, et al. Application of sustained delivery microsphere of cyclosporine A for preventing posterior capsular opacification in rabbits. Int J Ophthalmol. 2013;6(1):1-7doi: 10.3980/j.issn.2222-3959.2013.01.01.
Pei, C., Xu, Y., Jiang, J. X., Cui, L. J., Li, L., & Qin, L. (2013). Application of sustained delivery microsphere of cyclosporine A for preventing posterior capsular opacification in rabbits. International journal of ophthalmology, 6(1), 1-7. https://doi.org/10.3980/j.issn.2222-3959.2013.01.01
Pei, Cheng, et al. "Application of sustained delivery microsphere of cyclosporine A for preventing posterior capsular opacification in rabbits." International journal of ophthalmology vol. 6,1 (2013): 1-7. doi: https://doi.org/10.3980/j.issn.2222-3959.2013.01.01
Pei C, Xu Y, Jiang JX, Cui LJ, Li L, Qin L. Application of sustained delivery microsphere of cyclosporine A for preventing posterior capsular opacification in rabbits. Int J Ophthalmol. 2013;6(1):1-7. doi: 10.3980/j.issn.2222-3959.2013.01.01. Epub 2013 Feb 18. PMID: 23550193; PMCID: PMC3580240.
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Int J Ophthalmol. 2013;6(1):1-7. doi: 10.3980/j.issn.2222-3959.2013.01.01. Epub 2013 Feb 18.

Application of sustained delivery microsphere of cyclosporine A for preventing posterior capsular opacification in rabbits.

International journal of ophthalmology

Cheng Pei, Yi Xu, Jean Xin Jiang, Li-Jun Cui, Li Li, Li Qin

Affiliations

  1. Department of Ophthalmology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.

PMID: 23550193 PMCID: PMC3580240 DOI: 10.3980/j.issn.2222-3959.2013.01.01

Abstract

AIM: To explore the inhibitory effect of a sustained cyclosporin A (CsA) delivery microsphere (CsA-MS) on posterior capsular opacification (PCO) in rabbit eyes after cataract extraction.

METHODS: Twenty New Zealand white rabbits accepted cataract extraction plus intraocular lens implantation and their left eyes were intraoperatively injected CsA-MS prepared using polymer polylactioglycolic acid (PLGA) as a carrier and their right eyes were injected with empty MS. The changes in cornea, anterior chamber reaction, intraocular pressure, PCO and CsA concentration in aqueous humor were examined postoperatively and all the eyes were enucleated 3 months after surgery for histopathological and morphological examination with light microscopy and electron microscopy.

RESULTS: Conjunctival hyperemia, corneal edema, intraocular pressure and anterior chamber response of experimental and control eyes were similar, while PCO in CsA-MS injected eyes was greatly improved compared with that in control eyes. Posterior capsules in CsA-MS injected eyes were smooth and lens epithelial cells (LEC) did not proliferate significantly (P>0.05), while LEC in posterior capsule of control eyes had different degrees of proliferation and cortical regeneration. LEC in CsA-MS injected eyes were not functionally active and underwent apoptosis, whereas LEC in control eyes were functionally active (F-test, P=0.025). In addition, the corneal ultrastructure showed no differences between CsA-MS and MS injected eyes.

CONCLUSION: CsA-MS has high bioavailability in rabbit eyes and could inhibit postoperative PCO occurrence and development during the study period, suggesting that CsA-MS may be a promising, effective and safe administration route to prevent PCO in clinic.

Keywords: posterior capsular opacification; rabbit eyes; sustained cyclosporine A delivery microsphere

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