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Arul M, Roslani AC, Ng CL, et al. Culture of low passage colorectal cancer cells and demonstration of variation in selected tumour marker expression. Cytotechnology. 2013;66(3):481-91doi: 10.1007/s10616-013-9600-4.
Arul, M., Roslani, A. C., Ng, C. L., & Cheah, S. H. (2014). Culture of low passage colorectal cancer cells and demonstration of variation in selected tumour marker expression. Cytotechnology, 66(3), 481-91. https://doi.org/10.1007/s10616-013-9600-4
Arul, Melanie, et al. "Culture of low passage colorectal cancer cells and demonstration of variation in selected tumour marker expression." Cytotechnology vol. 66,3 (2014): 481-91. doi: https://doi.org/10.1007/s10616-013-9600-4
Arul M, Roslani AC, Ng CL, Cheah SH. Culture of low passage colorectal cancer cells and demonstration of variation in selected tumour marker expression. Cytotechnology. 2014 May;66(3):481-91. doi: 10.1007/s10616-013-9600-4. Epub 2013 Jul 04. PMID: 23824584; PMCID: PMC3973793.
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Cytotechnology. 2014 May;66(3):481-91. doi: 10.1007/s10616-013-9600-4. Epub 2013 Jul 04.

Culture of low passage colorectal cancer cells and demonstration of variation in selected tumour marker expression.

Cytotechnology

Melanie Arul, April Camilla Roslani, Colin Leong Liong Ng, Swee Hung Cheah

Affiliations

  1. Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, [email protected].

PMID: 23824584 PMCID: PMC3973793 DOI: 10.1007/s10616-013-9600-4

Abstract

There is increasing evidence that a tumour comprises of heterogeneous population of cells. Thus, studying homogenous cell lines in vitro may yield results that are not reflective of the true situation in a tumour and studying low passage cell lines maintained in a heterogeneous population before they transform away from the original state may provide a more complete picture of colorectal cancer. A method was developed to isolate and establish low passage colorectal cancer cell lines from tumour biopsies. The media contents, combination of antimicrobials and specimen collection and transport conditions employed, successfully eliminated microbial contamination which is frequently present in samples obtained from the gastrointestinal tract. A variety of growth forms indicating a heterogeneous mixture of cells was seen in the initial cultures. Using fluorescence immunocytochemistry, primary tumour cultures were shown to variably express selected tumour markers, carcinoembryonic antigen and C2 antigen. These low passage cell lines growing in a heterogeneous environment would more closely reflect the characteristics of the cells of the original tumour.

References

  1. Breast Cancer Res. 2003;5(2):89-95 - PubMed
  2. Cancer Res. 1984 Jun;44(6):2259-65 - PubMed
  3. J Natl Cancer Inst. 2008 May 7;100(9):672-9 - PubMed
  4. PLoS One. 2007 Apr 25;2(4):e394 - PubMed
  5. Cancer Metastasis Rev. 1983;2(1):5-23 - PubMed
  6. Tumori. 2009 May-Jun;95(3):343-7 - PubMed
  7. Eur J Cancer. 2007 Jun;43(9):1348-60 - PubMed
  8. Gastroenterology. 1989 Feb;96(2 Pt 1):283-91 - PubMed
  9. Cell. 2009 Sep 4;138(5):822-9 - PubMed
  10. Cancer Res. 2001 Feb 1;61(3):799-807 - PubMed
  11. Pharm Res. 1997 Jun;14(6):757-62 - PubMed
  12. Cell Cycle. 2007 Oct 1;6(19):2332-8 - PubMed
  13. Proc Soc Exp Biol Med. 1997 Mar;214(3):248-57 - PubMed
  14. Biosci Rep. 2004 Dec;24(6):631-9 - PubMed
  15. Breast Cancer Res Treat. 2004 Feb;83(3):249-89 - PubMed
  16. Cancer Lett. 1997 Feb 26;113(1-2):77-86 - PubMed
  17. Cell. 2010 Apr 2;141(1):69-80 - PubMed
  18. Biochim Biophys Acta. 2010 Jan;1805(1):105-17 - PubMed
  19. Br J Cancer. 1986 Jun;53(6):779-85 - PubMed
  20. PLoS One. 2010 Mar 23;5(3):e9832 - PubMed
  21. Mol Syst Biol. 2009;5:326 - PubMed
  22. PLoS One. 2011 Mar 30;6(3):e17866 - PubMed
  23. Cancer Res. 1989 Mar 1;49(5):1282-6 - PubMed
  24. Int J Cancer. 1984 Jul 15;34(1):49-56 - PubMed
  25. In Vitro Cell Dev Biol Anim. 2007 Mar-Apr;43(3-4):105-8 - PubMed
  26. Int J Cancer. 1999 Nov 12;83(4):555-63 - PubMed
  27. Cancer Res. 1986 May;46(5):2203-7 - PubMed
  28. J Steroid Biochem Mol Biol. 1997 Aug;62(5-6):391-9 - PubMed
  29. FASEB J. 1992 Jun;6(9):2726-34 - PubMed
  30. Cancer Cell. 2010 Nov 16;18(5):510-23 - PubMed
  31. Cancer Genomics Proteomics. 2009 Jan-Feb;6(1):19-29 - PubMed
  32. Expert Rev Mol Diagn. 2012 Jul;12(6):621-8 - PubMed
  33. Cancer Res. 1978 Sep;38(9):2651-60 - PubMed
  34. Cell Biol Toxicol. 2005 Jan;21(1):1-26 - PubMed
  35. Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9276-81 - PubMed
  36. Semin Cancer Biol. 1999 Apr;9(2):67-81 - PubMed
  37. Methods Find Exp Clin Pharmacol. 2004 Nov;26(9):687-96 - PubMed
  38. Nat Rev Cancer. 2012 Apr 19;12(5):323-34 - PubMed

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