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Wu T, Zhang C, Wang Z, et al. Inhibitory role of S-nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins. Exp Ther Med. 2013;6(3):831-839doi: 10.3892/etm.2013.1221.
Wu, T., Zhang, C., Wang, Z., Liu, J., Li, H., Zhou, W., & Wang, S. (2013). Inhibitory role of S-nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins. Experimental and therapeutic medicine, 6(3), 831-839. https://doi.org/10.3892/etm.2013.1221
Wu, Tao, et al. "Inhibitory role of S-nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins." Experimental and therapeutic medicine vol. 6,3 (2013): 831-839. doi: https://doi.org/10.3892/etm.2013.1221
Wu T, Zhang C, Wang Z, Liu J, Li H, Zhou W, Wang S. Inhibitory role of S-nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins. Exp Ther Med. 2013 Sep;6(3):831-839. doi: 10.3892/etm.2013.1221. Epub 2013 Jul 12. PMID: 24137275; PMCID: PMC3786807.
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Exp Ther Med. 2013 Sep;6(3):831-839. doi: 10.3892/etm.2013.1221. Epub 2013 Jul 12.

Inhibitory role of S-nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins.

Experimental and therapeutic medicine

Tao Wu, Changhong Zhang, Zantao Wang, Jinghan Liu, Hui Li, Wu Zhou, Shuying Wang

Affiliations

  1. Department of Blood Transfusion, General Hospital of Beijing Military Region, Beijing 100700, P.R. China.

PMID: 24137275 PMCID: PMC3786807 DOI: 10.3892/etm.2013.1221

Abstract

The aim of this study was to explore the effects of S-nitrosoglutathione (GSNO) on the aggregation of frozen platelets, the platelet nitric oxide (NO) content and the expression of membrane glycoproteins. The level of frozen platelet aggregation was measured using a platelet aggregation analyzer, while the content of NO was measured by the nitrate reductase method and the expression of frozen platelet membrane glycoproteins was determined by flow cytometry. The level of frozen platelet aggregation was reduced from 35.47±2.93 to 24.43±3.07% following treatment with GSNO. The mean NO concentration in the 32 samples of frozen platelets treated with GSNO was 45.64±6.31 μmol/l, which was significantly higher compared with the concentration in the fresh liquid platelet group. There were no significant differences in the levels of PAC-1 in the fresh liquid platelet, frozen platelet and GSNO-treated frozen platelet groups; by contrast, significant differences were observed in the CD42b and CD62P levels. The platelet membrane glycoprotein expression levels in the frozen platelet and the GSNO-treated frozen platelet groups were not significantly different. The results of the study indicate that GSNO has potential as a cryoprotectant, due to its ability to increase the NO concentration in frozen platelets, inhibit platelet aggregation and maintain platelet function. It is likely that the molecular arrangement and structure of the frozen platelets were altered following GSNO treatment, or that the frozen platelets were affected by alternative mechanisms.

Keywords: S-nitrosoglutathione; aggregation; frozen platelets; membrane glycoprotein molecules; nitric oxide

References

  1. Br J Pharmacol. 2010 Apr;159(8):1572-80 - PubMed
  2. Blood. 2011 Sep 29;118(13):3670-9 - PubMed
  3. Korean J Lab Med. 2008 Apr;28(2):136-43 - PubMed
  4. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Apr;18(2):490-3 - PubMed
  5. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Dec;15(6):1284-8 - PubMed
  6. Biochem Biophys Res Commun. 1993 Jul 30;194(2):960-5 - PubMed
  7. Vox Sang. 2007 Jul;93(1):27-36 - PubMed
  8. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Oct;16(5):1188-91 - PubMed
  9. Platelets. 2009 Nov;20(7):478-86 - PubMed
  10. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Aug;16(4):930-2 - PubMed
  11. Biomaterials. 2010 Apr;31(10):2736-45 - PubMed
  12. Perfusion. 2007 May;22(3):193-200 - PubMed
  13. Nitric Oxide. 2011 Oct 30;25(3):303-8 - PubMed
  14. Curr Drug Targets. 2012 Aug;13(9):1207-14 - PubMed
  15. Methods Mol Biol. 2011;704:27-37 - PubMed
  16. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Jun;27(6):650-2 - PubMed
  17. Transfus Apher Sci. 2004 Feb;30(1):29-39 - PubMed

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