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Halldén C, Hansen M, Nilsson NO, et al. Competition as a source of errors in RAPD analysis. Theor Appl Genet. 1996;93(8):1185-92doi: 10.1007/BF00223449.
Halldén, C., Hansen, M., Nilsson, N. O., Hjerdin, A., & Säll, T. (1996). Competition as a source of errors in RAPD analysis. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 93(8), 1185-92. https://doi.org/10.1007/BF00223449
Halldén, C, et al. "Competition as a source of errors in RAPD analysis." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 93,8 (1996): 1185-92. doi: https://doi.org/10.1007/BF00223449
Halldén C, Hansen M, Nilsson NO, Hjerdin A, Säll T. Competition as a source of errors in RAPD analysis. Theor Appl Genet. 1996 Dec;93(8):1185-92. doi: 10.1007/BF00223449. PMID: 24162529.
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Theor Appl Genet. 1996 Dec;93(8):1185-92. doi: 10.1007/BF00223449.

Competition as a source of errors in RAPD analysis.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

C Halldén, M Hansen, N O Nilsson, A Hjerdin, T Säll

Affiliations

  1. Hilleshög AB, Box 302, S-261 23, Landskrona, Sweden.

PMID: 24162529 DOI: 10.1007/BF00223449

Abstract

We have used artificial 1∶1 DNA mixtures of all pairwise combinations of four doubled haploid Brassica napus lines to test the ability of RAPDs to function as reliable dominant genetic markers. In situations where a specific RAPD band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1∶1 DNA mixture. In 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the RAPD reaction. Thus, RAPD markers will lead to an erroneous genetic interpretation in 14% of all cases. In contrast, the formation of non-parental heteroduplex bands was found at a frequency of only 0.2%. Analysis of 1∶ 1 mixtures using (1) a different set of optimized reaction conditions and (2) a material with low genomic complexity (Bacillus cereus) gave identical results. Serial dilutions of one genome into another, in steps of 10%, showed that all of the polymorphic bands decreased in intensity as a linear function of their respective proportion in the mixture. In dilutions with water no differences in band intensity were detected. Thus, competition occurs in the amplification of all RAPD fragments and is a major source of genotyping errors in RAPD analysis.

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