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Terakawa T, Sato T, Koike M. Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.). Plant Cell Rep. 1992;11(9):457-61doi: 10.1007/BF00232690.
Terakawa, T., Sato, T., & Koike, M. (1992). Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.). Plant cell reports, 11(9), 457-61. https://doi.org/10.1007/BF00232690
Terakawa, T, et al. "Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.)." Plant cell reports vol. 11,9 (1992): 457-61. doi: https://doi.org/10.1007/BF00232690
Terakawa T, Sato T, Koike M. Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.). Plant Cell Rep. 1992 Aug;11(9):457-61. doi: 10.1007/BF00232690. PMID: 24201589.
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Plant Cell Rep. 1992 Aug;11(9):457-61. doi: 10.1007/BF00232690.

Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.).

Plant cell reports

T Terakawa, T Sato, M Koike

Affiliations

  1. Central Research Laboratories, Hokko Chemical Industry Co., Ltd., 2165 Toda, Atsugi, 243, Kanagawa, Japan.

PMID: 24201589 DOI: 10.1007/BF00232690

Abstract

A rapidly growing embryogenic suspension culture cell line of creeping bentgrass cv Penncross (Agrostis palustris Huds.) was established from callus derived from the culture of mature seeds. High concentrations of 2,4-D were required for the induction of callus (3 mg/1) as well as for the maintenance of the cell Une (2 mg/1) on modified B5 medium of Gamborg. Protoplasts isolated from the suspension cultures were successfully cultured in Murashige and Skoog's basal medium supplemented with only 0.1 mg/1 2,4-D. Although protoplast plating efficiency was rather low (0.36%), 30% of the protocalli formed normal green plants that were successfully established in soil.

References

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