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Grad M, Young EF, Smilenov L, et al. A simple add-on microfluidic appliance for accurately sorting small populations of cells with high fidelity. J Micromech Microeng. 2013;23(11)doi: 10.1088/0960-1317/23/11/117003.
Grad, M., Young, E. F., Smilenov, L., Brenner, D. J., & Attinger, D. (2013). A simple add-on microfluidic appliance for accurately sorting small populations of cells with high fidelity. Journal of micromechanics and microengineering : structures, devices, and systems, 23(11), . https://doi.org/10.1088/0960-1317/23/11/117003
Grad, Michael, et al. "A simple add-on microfluidic appliance for accurately sorting small populations of cells with high fidelity." Journal of micromechanics and microengineering : structures, devices, and systems vol. 23,11 (2013). doi: https://doi.org/10.1088/0960-1317/23/11/117003
Grad M, Young EF, Smilenov L, Brenner DJ, Attinger D. A simple add-on microfluidic appliance for accurately sorting small populations of cells with high fidelity. J Micromech Microeng. 2013;23(11). doi: 10.1088/0960-1317/23/11/117003. PMID: 24409041; PMCID: PMC3883503.
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J Micromech Microeng. 2013;23(11). doi: 10.1088/0960-1317/23/11/117003.

A simple add-on microfluidic appliance for accurately sorting small populations of cells with high fidelity.

Journal of micromechanics and microengineering : structures, devices, and systems

Michael Grad, Erik F Young, Lubomir Smilenov, David J Brenner, Daniel Attinger

Affiliations

  1. Department of Mechanical Engineering, Columbia University ; Center for Radiological Research, Columbia University.
  2. Center for Radiological Research, Columbia University.
  3. Department of Mechanical Engineering, Iowa State University.

PMID: 24409041 PMCID: PMC3883503 DOI: 10.1088/0960-1317/23/11/117003

Abstract

Current advances in single cell sequencing, gene expression and proteomics require the isolation of single cells, frequently from a very small source population. In this work we describe the design and characterization of a manually operated microfluidic cell sorter that 1) can accurately sort single or small groups of cells from very small cell populations with minimal losses, 2) that is easy to operate and that can be used in any laboratory that has a basic fluorescent microscope and syringe pump, 3) that can be assembled within minutes, 4) that can sort cells in very short time (minutes) with minimum cell stress, 5) that is cheap and reusable. This microfluidic sorter is made from hard plastic material (PMMA) into which microchannels are directly milled with hydraulic diameter of 70 μm. Inlet and outlet reservoirs are drilled through the chip. Sorting occurs through hydrodynamic switching ensuring low hydrodynamic shear stresses, which were modeled or experimentally confirmed to be below the cell damage threshold. Manually operated, the maximum sorting frequencies were approximately 10 cells per minute. Experiments verified that cell sorting operations could be achieved in as little as 15 minutes, including the assembly and testing of the sorter. In only one out of 10 sorting experiments the sorted cells were contaminated with another cell type. This microfluidic cell sorter represents an important capability for protocols requiring fast isolation of single cells from small number of rare cell populations.

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