Display options
Cite
Popovic M, Andjelkovic U, Grozdanovic M, et al. In Vitro Antibacterial Activity of Cysteine Protease Inhibitor from Kiwifruit (Actinidia deliciosa). Indian J Microbiol. 2012;53(1):100-5doi: 10.1007/s12088-012-0319-2.
Popovic, M., Andjelkovic, U., Grozdanovic, M., Aleksic, I., & Gavrovic-Jankulovic, M. (2013). In Vitro Antibacterial Activity of Cysteine Protease Inhibitor from Kiwifruit (Actinidia deliciosa). Indian journal of microbiology, 53(1), 100-5. https://doi.org/10.1007/s12088-012-0319-2
Popovic, Milica, et al. "In Vitro Antibacterial Activity of Cysteine Protease Inhibitor from Kiwifruit (Actinidia deliciosa)." Indian journal of microbiology vol. 53,1 (2013): 100-5. doi: https://doi.org/10.1007/s12088-012-0319-2
Popovic M, Andjelkovic U, Grozdanovic M, Aleksic I, Gavrovic-Jankulovic M. In Vitro Antibacterial Activity of Cysteine Protease Inhibitor from Kiwifruit (Actinidia deliciosa). Indian J Microbiol. 2013 Mar;53(1):100-5. doi: 10.1007/s12088-012-0319-2. Epub 2012 Oct 19. PMID: 24426085; PMCID: PMC3587517.
Copy Download .nbib Format: NLM
Share it on

Indian J Microbiol. 2013 Mar;53(1):100-5. doi: 10.1007/s12088-012-0319-2. Epub 2012 Oct 19.

In Vitro Antibacterial Activity of Cysteine Protease Inhibitor from Kiwifruit (Actinidia deliciosa).

Indian journal of microbiology

Milica Popovic, Uros Andjelkovic, Milica Grozdanovic, Ivana Aleksic, Marija Gavrovic-Jankulovic

Affiliations

  1. Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
  2. Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
  3. Institute of Virology, Vaccines and Sera Torlak, Belgrade, Serbia.

PMID: 24426085 PMCID: PMC3587517 DOI: 10.1007/s12088-012-0319-2

Abstract

The need for replacing traditional pesticides with alternative agents for the management of agricultural pathogens is rising worldwide. In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. We examined the growth inhibition of three plant pathogenic Gram-negative bacterial strains by kiwi CPI and attempted to elucidate the potential mechanism of the growth inhibition. CPI influenced the growth of phytopathogenic bacteria Agrobacterium tumefaciens (76.2 % growth inhibition using 15 μM CPI), Burkholderia cepacia (75.6 % growth inhibition) and, to a lesser extent, Erwinia carotovora (44.4 % growth inhibition) by inhibiting proteinases that are excreted by these bacteria. Identification and characterization of natural plant defense molecules is the first step toward creation of improved methods for pest control based on naturally occurring molecules.

Keywords: Antibacterial; Cysteine protease inhibitor; Kiwifruit cystatin; Phytocystatin; Plant natural resistance

References

  1. Mol Nutr Food Res. 2010 Mar;54(3):373-80 - PubMed
  2. Parasitology. 1997 Jun;114 ( Pt 6):605-13 - PubMed
  3. Scand J Immunol Suppl. 1973;1:161-4 - PubMed
  4. Biosci Biotechnol Biochem. 2001 Jan;65(1):22-8 - PubMed
  5. Food Addit Contam. 2005 May;22(5):423-8 - PubMed
  6. Plant Cell. 1996 Oct;8(10):1651-1668 - PubMed
  7. Plant Signal Behav. 2012 Oct 1;7(10):1306-20 - PubMed
  8. Phytochemistry. 2004 Jan;65(1):19-30 - PubMed
  9. Biochem J. 1983 Apr 1;211(1):129-38 - PubMed
  10. J Exp Bot. 2005 Jul;56(417):1821-9 - PubMed
  11. Biochimie. 2010 Nov;92(11):1657-66 - PubMed
  12. Curr Protein Pept Sci. 2011 Aug;12(5):386-98 - PubMed
  13. Microbes Infect. 2002 Apr;4(4):405-12 - PubMed
  14. Arch Biochem Biophys. 1998 Nov 1;359(1):24-30 - PubMed
  15. Eur J Biochem. 1992 Nov 1;209(3):933-7 - PubMed
  16. Plant Mol Biol. 1998 Dec;38(6):1235-42 - PubMed
  17. Plant Biotechnol J. 2010 Jan;8(1):65-75 - PubMed

Publication Types