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Lu Y, Zhou W, Wei L, et al. Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica. Biotechnol Biofuels. 2014;7:81doi: 10.1186/1754-6834-7-81.
Lu, Y., Zhou, W., Wei, L., Li, J., Jia, J., Li, F., Smith, S. M., & Xu, J. (2014). Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica. Biotechnology for biofuels, 781. https://doi.org/10.1186/1754-6834-7-81
Lu, Yandu, et al. "Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica." Biotechnology for biofuels vol. 7 (2014): 81. doi: https://doi.org/10.1186/1754-6834-7-81
Lu Y, Zhou W, Wei L, Li J, Jia J, Li F, Smith SM, Xu J. Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica. Biotechnol Biofuels. 2014 May 30;7:81. doi: 10.1186/1754-6834-7-81. eCollection 2014. PMID: 24920959; PMCID: PMC4052811.
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Biotechnol Biofuels. 2014 May 30;7:81. doi: 10.1186/1754-6834-7-81. eCollection 2014.

Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica.

Biotechnology for biofuels

Yandu Lu, Wenxu Zhou, Li Wei, Jing Li, Jing Jia, Fei Li, Steven M Smith, Jian Xu

Affiliations

  1. Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China.
  2. Australian Research Council, Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

PMID: 24920959 PMCID: PMC4052811 DOI: 10.1186/1754-6834-7-81

Abstract

BACKGROUND: Sterols are vital structural and regulatory components in eukaryotic cells; however, their biosynthetic pathways and functional roles in microalgae remain poorly understood.

RESULTS: In the oleaginous microalga Nannochloropsis oceanica, the sterol biosynthetic pathway produces phytosterols as minor products and cholesterol as the major product. The evidence together with their deduced biosynthetic pathways suggests that N. oceanica exhibits features of both higher plants and mammals. Temporal tracking of sterol profiles and sterol-biosynthetic transcripts in response to changes in light intensity and nitrogen supply reveal that sterols play roles in cell proliferation, chloroplast differentiation, and photosynthesis. Furthermore, the dynamics of fatty acid (FA) and FA-biosynthetic transcripts upon chemical inhibitor-induced sterol depletion reveal possible co-regulation of sterol production and FA synthesis, in that the squalene epoxidase inhibitor terbinafine reduces sterol content yet significantly elevates free FA production. Thus, a feedback regulation of sterol and FA homeostasis is proposed, with the 1-deoxy-D-xylulose 5-phosphate synthase (DXS, the committed enzyme in isoprenoid and sterol biosynthesis) gene potentially subject to feedback regulation by sterols.

CONCLUSION: These findings reveal features of sterol function and biosynthesis in microalgae and suggest new genetic engineering or chemical biology approaches for enhanced oil production in microalgae.

Keywords: Cholesterol biosynthetic pathway; Fatty acid biosynthesis; Feedback regulation; Nannochloropsis

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