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Gomes LC, Moreira JM, Simões M, et al. Biofilm localization in the vertical wall of shaking 96-well plates. Scientifica (Cairo). 2014;2014:231083doi: 10.1155/2014/231083.
Gomes, L. C., Moreira, J. M., Simões, M., Melo, L. F., & Mergulhão, F. J. (2014). Biofilm localization in the vertical wall of shaking 96-well plates. Scientifica, 2014231083. https://doi.org/10.1155/2014/231083
Gomes, Luciana C, et al. "Biofilm localization in the vertical wall of shaking 96-well plates." Scientifica vol. 2014 (2014): 231083. doi: https://doi.org/10.1155/2014/231083
Gomes LC, Moreira JM, Simões M, Melo LF, Mergulhão FJ. Biofilm localization in the vertical wall of shaking 96-well plates. Scientifica (Cairo). 2014;2014:231083. doi: 10.1155/2014/231083. Epub 2014 Apr 13. PMID: 24834360; PMCID: PMC4009116.
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Scientifica (Cairo). 2014;2014:231083. doi: 10.1155/2014/231083. Epub 2014 Apr 13.

Biofilm localization in the vertical wall of shaking 96-well plates.

Scientifica

Luciana C Gomes, Joana M R Moreira, Manuel Simões, Luís F Melo, Filipe J Mergulhão

Affiliations

  1. LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.

PMID: 24834360 PMCID: PMC4009116 DOI: 10.1155/2014/231083

Abstract

Microtiter plates with 96 wells are being increasingly used for biofilm studies due to their high throughput, low cost, easy handling, and easy application of several analytical methods to evaluate different biofilm parameters. These methods provide bulk information about the biofilm formed in each well but lack in detail, namely, regarding the spatial location of the biofilms. This location can be obtained by microscopy observation using optical and electron microscopes, but these techniques have lower throughput and higher cost and are subjected to equipment availability. This work describes a differential crystal violet (CV) staining method that enabled the determination of the spatial location of Escherichia coli biofilms formed in the vertical wall of shaking 96-well plates. It was shown that the biofilms were unevenly distributed on the wall with denser cell accumulation near the air-liquid interface. The results were corroborated by scanning electron microscopy and a correlation was found between biofilm accumulation and the wall shear strain rates determined by computational fluid dynamics. The developed method is quicker and less expensive and has a higher throughput than the existing methods available for spatial location of biofilms in microtiter plates.

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