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Nakamura T, Nagura T, Sato K, et al. Evaluation of the effects of dietary organic germanium, ge-132, and raffinose supplementation on caecal flora in rats. Biosci Microbiota Food Health. 2012;31(2):37-45doi: 10.12938/bmfh.31.37.
Nakamura, T., Nagura, T., Sato, K., & Ohnishi, M. (2012). Evaluation of the effects of dietary organic germanium, ge-132, and raffinose supplementation on caecal flora in rats. Bioscience of microbiota, food and health, 31(2), 37-45. https://doi.org/10.12938/bmfh.31.37
Nakamura, Takashi, et al. "Evaluation of the effects of dietary organic germanium, ge-132, and raffinose supplementation on caecal flora in rats." Bioscience of microbiota, food and health vol. 31,2 (2012): 37-45. doi: https://doi.org/10.12938/bmfh.31.37
Nakamura T, Nagura T, Sato K, Ohnishi M. Evaluation of the effects of dietary organic germanium, ge-132, and raffinose supplementation on caecal flora in rats. Biosci Microbiota Food Health. 2012;31(2):37-45. doi: 10.12938/bmfh.31.37. Epub 2012 Apr 20. PMID: 24936347; PMCID: PMC4034287.
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Biosci Microbiota Food Health. 2012;31(2):37-45. doi: 10.12938/bmfh.31.37. Epub 2012 Apr 20.

Evaluation of the effects of dietary organic germanium, ge-132, and raffinose supplementation on caecal flora in rats.

Bioscience of microbiota, food and health

Takashi Nakamura, Taizo Nagura, Katsuyuki Sato, Masao Ohnishi

Affiliations

  1. Asai Germanium Research Institute Co., Ltd., 3-131 Suzuranoka, Hakodate, Hokkaido 042-0958, Japan.
  2. Nippon Beet Sugar Mfg. Co., Ltd., Nishi 13, Minami 9 Sen, Inada, Obihiro, Hokkaido 080-0831, Japan.
  3. Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 Sen, Inada, Obihiro, Hokkaido 080-8555, Japan.

PMID: 24936347 PMCID: PMC4034287 DOI: 10.12938/bmfh.31.37

Abstract

Poly-trans-[(2-carboxyethyl) germasesquioxane] (Ge-132) is the most common organic germanium compound. The ingestion of Ge-132 promotes bile secretion. We assessed the rat caecal characteristics after the administration of Ge-132 and raffinose, a prebiotic oligosaccharide, because both Ge-132 and some prebiotics can change the fecal color to yellow. We also compared the changes in the caecal flora caused by the two compounds. In addition, we evaluated the simultaneous administration of Ge-132 and raffinose and their effects on β-glucuronidase activity, which is known to be a factor related to colon cancer. Male Wistar rats (three weeks old) were given one of the following diets: 1) a control diet (control group), 2) a diet containing 0.05% Ge-132 (Ge-132 group), 3) a diet containing 5% raffinose (RAF group) or 4) a diet containing 0.05% Ge-132 + 5% raffinose (GeRAF group). The Bifidobacterium, Lactobacillus and total bacteria counts were significantly increased by the dietary raffinose, and Ge-132 did not suppress this increase. The raffinose intake increased caecal acetic acid production significantly. The activity of β-glucuronidase in the caecal contents was increased by dietary Ge-132, whereas dietary raffinose decreased the β-glucuronidase activity significantly. These results indicate that the simultaneous intake of dietary raffinose and Ge-132 does not inhibit the effects of either compound on intestinal fermentation and bile secretion. Additionally, the simultaneous intake of both raffinose and Ge-132 could abrogate the increase in β-glucuronidase activity induced by Ge-132 alone.

Keywords: Bifidobacterium; Ge-132; caecal flora; organic germanium; raffinose; β-glucuronidase

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