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Eren Y, Erdoğmuş SF, Akyıl D, et al. Cytotoxic and genotoxic effects of dioxacarb by human peripheral blood lymphocytes CAs and Allium test. Cytotechnology. 2014;67(6):1023-30doi: 10.1007/s10616-014-9741-0.
Eren, Y., Erdoğmuş, S. F., Akyıl, D., Özkara, A., Konuk, M., & Sağlam, E. (2015). Cytotoxic and genotoxic effects of dioxacarb by human peripheral blood lymphocytes CAs and Allium test. Cytotechnology, 67(6), 1023-30. https://doi.org/10.1007/s10616-014-9741-0
Eren, Yasin, et al. "Cytotoxic and genotoxic effects of dioxacarb by human peripheral blood lymphocytes CAs and Allium test." Cytotechnology vol. 67,6 (2015): 1023-30. doi: https://doi.org/10.1007/s10616-014-9741-0
Eren Y, Erdoğmuş SF, Akyıl D, Özkara A, Konuk M, Sağlam E. Cytotoxic and genotoxic effects of dioxacarb by human peripheral blood lymphocytes CAs and Allium test. Cytotechnology. 2015 Dec;67(6):1023-30. doi: 10.1007/s10616-014-9741-0. Epub 2014 May 22. PMID: 24848210; PMCID: PMC4628927.
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Cytotechnology. 2015 Dec;67(6):1023-30. doi: 10.1007/s10616-014-9741-0. Epub 2014 May 22.

Cytotoxic and genotoxic effects of dioxacarb by human peripheral blood lymphocytes CAs and Allium test.

Cytotechnology

Yasin Eren, Sevim Feyza Erdoğmuş, Dilek Akyıl, Arzu Özkara, Muhsin Konuk, Esra Sağlam

Affiliations

  1. Department of Science Education, Faculty of Education, Süleyman Demirel University, 32260, Isparta, Turkey. [email protected].
  2. Department of Laboratory and Veterinary Health, Bayat Vocational School, Afyon Kocatepe University, Afyon, Turkey.
  3. Department of Biology, Faculty of Science and Arts, Afyon Kocatepe University, Afyon, Turkey.
  4. Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey.
  5. Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Üsküdar University, Istanbul, Turkey.

PMID: 24848210 PMCID: PMC4628927 DOI: 10.1007/s10616-014-9741-0

Abstract

Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and genotoxic effects of this pesticide on human peripheral blood lymphocytes and Allium root meristematic cells were investigated by chromosomal aberrations (CAs) and Allium test. Human lymphocytes were treated with 62.5, 125, 250 and 500 ppm doses of dioxacarb for CAs. CA/cell, abnormal cell % and mitotic index % (MI %) data were obtained from these concentrations in 24 and 48 h treatment periods. Dioxacarb did not increase the CA/cell frequency significantly, so this insecticide was not identified as genotoxic. But it was found cytotoxic especially at 250 and 500 ppm concentrations because of the reduced the MI % and increased the abnormal cell %. In Allium test, 25 ppm (EC50/2), 50 ppm (EC50) and 100 ppm (EC50 × 2) concentrations were used for root growth inhibition (EC50 determination) and Allium mitotic index (MI) determination tests. The used concentrations of dioxacarb induced dose-dependent inhibition of MI and root growth on root meristems. Mitotic inhibition of dioxacarb was found significantly higher than for the positive control. These Allium results indicated the high cytotoxicity of dioxacarb. The present study is the first research on cytotoxicity and genotoxicity of dioxacarb by human lymphocyte CAs and Allium test.

Keywords: Allium test; CAs; Cytotoxicity; Dioxacarb; Genotoxicity

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