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Lin Y, Murray MA, Garrett IR, et al. A targeted approach for evaluating preclinical activity of botanical extracts for support of bone health. J Nutr Sci. 2014;3:e13doi: 10.1017/jns.2014.5.
Lin, Y., Murray, M. A., Garrett, I. R., Gutierrez, G. E., Nyman, J. S., Mundy, G., Fast, D., Gellenbeck, K. W., Chandra, A., & Ramakrishnan, S. (2014). A targeted approach for evaluating preclinical activity of botanical extracts for support of bone health. Journal of nutritional science, 3e13. https://doi.org/10.1017/jns.2014.5
Lin, Yumei, et al. "A targeted approach for evaluating preclinical activity of botanical extracts for support of bone health." Journal of nutritional science vol. 3 (2014): e13. doi: https://doi.org/10.1017/jns.2014.5
Lin Y, Murray MA, Garrett IR, Gutierrez GE, Nyman JS, Mundy G, Fast D, Gellenbeck KW, Chandra A, Ramakrishnan S. A targeted approach for evaluating preclinical activity of botanical extracts for support of bone health. J Nutr Sci. 2014 May 13;3:e13. doi: 10.1017/jns.2014.5. eCollection 2014. PMID: 25191605; PMCID: PMC4153014.
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J Nutr Sci. 2014 May 13;3:e13. doi: 10.1017/jns.2014.5. eCollection 2014.

A targeted approach for evaluating preclinical activity of botanical extracts for support of bone health.

Journal of nutritional science

Yumei Lin, Mary A Murray, I Ross Garrett, Gloria E Gutierrez, Jeffry S Nyman, Gregory Mundy, David Fast, Kevin W Gellenbeck, Amitabh Chandra, Shyam Ramakrishnan

Affiliations

  1. Nutrilite Health Institute , 5600 Beach Boulevard, Buena Park, CA 90622 , USA.
  2. OsteoScreen Ltd , 2040 Babcock Road, San Antonio, TX 78023 , USA ; 9909 Charthouse Cove, Austin, TX 78730 , USA.
  3. OsteoScreen Ltd , 2040 Babcock Road, San Antonio, TX 78023 , USA ; Southwest Research Institute , 6220 Culebra Road, San Antonio, TX 78238 , USA.
  4. OsteoScreen Ltd , 2040 Babcock Road, San Antonio, TX 78023 , USA ; Department of Orthopaedic Surgery and Rehabilitation , Vanderbilt University Medical Center , Nashville, TN 37232 , USA.
  5. OsteoScreen Ltd , 2040 Babcock Road, San Antonio, TX 78023 , USA ; Vanderbilt University Medical Center , Nashville, TN 37232 , USA.
  6. Access Business Group , 7575 East Fulton Avenue, Ada, MI 49355 , USA.
  7. Nutrilite Health Institute , 5600 Beach Boulevard, Buena Park, CA 90622 , USA ; The Himalaya Drug Company , Makali, Tumkur Road, Bangalore - 562123 , India.

PMID: 25191605 PMCID: PMC4153014 DOI: 10.1017/jns.2014.5

Abstract

Using a sequential in vitro/in vivo approach, we tested the ability of botanical extracts to influence biomarkers associated with bone resorption and bone formation. Pomegranate fruit and grape seed extracts were found to exhibit anti-resorptive activity by inhibiting receptor activator of nuclear factor-κB ligand (RANKL) expression in MG-63 cells and to reduce IL-1β-stimulated calvarial (45)Ca loss. A combination of pomegranate fruit and grape seed extracts were shown to be effective at inhibiting bone loss in ovariectomised rats as demonstrated by standard histomorphometry, biomechanical and bone mineral density measurements. Quercetin and licorice extract exhibited bone formation activity as measured by bone morphogenetic protein-2 (BMP-2) promoter activation, increased expression of BMP-2 mRNA and protein levels, and promotion of bone growth in cultured mouse calvariae. A combination of quercetin and licorice extract demonstrated a potential for increasing bone mineral density in an intact female rat model as compared with controls. The results from this sequential in vitro/in vivo research model yielded botanical extract formulas that demonstrate significant potential benefits for bone health.

Keywords: AR, anti-resorptive sample; BF, bone formation sample; BMD, bone mineral density; BMP, bone morphogenetic protein; Bone formation; Bone morphogenetic protein-2; Botanical extracts; OVX, ovariectomised; PTH, parathyroid hormone; RANKL, receptor activator of nuclear factor-κB ligand; Receptor activator of nuclear factor-κB ligand; SHAM, sham-operated; vBMD, volumetric bone mineral density; µCT, micro-computed tomography device

References

  1. J Bone Miner Res. 2009 Feb;24(2):209-20 - PubMed
  2. J Steroid Biochem Mol Biol. 2004 Aug;91(4-5):241-6 - PubMed
  3. J Ethnopharmacol. 2004 May;92(1):93-101 - PubMed
  4. Eur J Oral Sci. 2005 Feb;113(1):47-52 - PubMed
  5. Biochem Pharmacol. 2005 Aug 1;70(3):363-8 - PubMed
  6. Am J Physiol Renal Physiol. 2003 Jan;284(1):F32-40 - PubMed
  7. Trends Mol Med. 2009 Sep;15(9):417-29 - PubMed
  8. Clin Biochem. 2012 Aug;45(12):887-93 - PubMed
  9. Biosci Biotechnol Biochem. 2002 Jul;66(7):1479-87 - PubMed
  10. Nutrition. 2008 Jul-Aug;24(7-8):733-43 - PubMed
  11. Br J Nutr. 2008 Apr;99(4):715-22 - PubMed
  12. J Clin Invest. 1990 Mar;85(3):632-9 - PubMed
  13. J Bone Miner Res. 2012 Sep;27(9):1872-8 - PubMed
  14. Mol Nutr Food Res. 2005 Aug;49(8):763-71 - PubMed
  15. Biochem Pharmacol. 2004 Apr 1;67(7):1307-13 - PubMed
  16. Breast Cancer Res Treat. 2002 Feb;71(3):203-17 - PubMed
  17. Altern Med Rev. 1999 Feb;4(1):10-22 - PubMed
  18. Endocrinology. 1996 Jan;137(1):331-9 - PubMed
  19. Int J Mol Med. 2011 Oct;28(4):521-5 - PubMed
  20. J Nutr. 2005 Sep;135(9):2096-102 - PubMed
  21. Br J Nutr. 2012 Mar;107(5):683-90 - PubMed
  22. Science. 1999 Dec 3;286(5446):1946-9 - PubMed
  23. Bone. 1988;9(1):67-9 - PubMed
  24. Am J Clin Nutr. 1999 Apr;69(4):727-36 - PubMed
  25. Cochrane Database Syst Rev. 2010 Jun 16;(6):CD006950 - PubMed
  26. Am J Clin Nutr. 2005 Feb;81(2):341-54 - PubMed
  27. Immunol Lett. 2009 Jun 4;124(2):102-10 - PubMed
  28. Methods Mol Med. 2003;80:183-98 - PubMed
  29. J Agric Food Chem. 2006 Nov 15;54(23):8956-61 - PubMed
  30. Bone. 2003 Feb;32(2):127-35 - PubMed
  31. Am J Clin Nutr. 2000 Jan;71(1):142-51 - PubMed
  32. J Bone Miner Metab. 2009;27(6):673-81 - PubMed
  33. J Musculoskelet Neuronal Interact. 2005 Jun;5(2):162-9 - PubMed
  34. Clin Pharmacokinet. 2010;49(2):89-118 - PubMed

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