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Hamada T. Lessons from in vitro reconstitution analyses of plant microtubule-associated proteins. Front Plant Sci. 2014;5:409doi: 10.3389/fpls.2014.00409.
Hamada, T. (2014). Lessons from in vitro reconstitution analyses of plant microtubule-associated proteins. Frontiers in plant science, 5409. https://doi.org/10.3389/fpls.2014.00409
Hamada, Takahiro. "Lessons from in vitro reconstitution analyses of plant microtubule-associated proteins." Frontiers in plant science vol. 5 (2014): 409. doi: https://doi.org/10.3389/fpls.2014.00409
Hamada T. Lessons from in vitro reconstitution analyses of plant microtubule-associated proteins. Front Plant Sci. 2014 Aug 22;5:409. doi: 10.3389/fpls.2014.00409. eCollection 2014. PMID: 25202315; PMCID: PMC4141329.
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Front Plant Sci. 2014 Aug 22;5:409. doi: 10.3389/fpls.2014.00409. eCollection 2014.

Lessons from in vitro reconstitution analyses of plant microtubule-associated proteins.

Frontiers in plant science

Takahiro Hamada

Affiliations

  1. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro, Japan.

PMID: 25202315 PMCID: PMC4141329 DOI: 10.3389/fpls.2014.00409

Abstract

Plant microtubules, composed of tubulin GTPase, are irreplaceable cellular components that regulate the directions of cell expansion and cell division, chromosome segregation and cell plate formation. To accomplish these functions, plant cells organize microtubule structures by regulating microtubule dynamics. Each microtubule localizes to the proper position with repeated growth and shortening. Although it is possible to reconstitute microtubule dynamics with pure tubulin solution in vitro, many microtubule-associated proteins (MAPs) govern microtubule dynamics in cells. In plants, major MAPs are identified as microtubule stabilizers (CLASP and MAP65 etc.), microtubule destabilizers (kinesin-13, katanin, MAP18 and MDP25), and microtubule dynamics promoters (EB1, MAP215, MOR1, MAP200, SPR2). Mutant analyses with forward and reverse genetics have shown the importance of microtubules and individual MAPs in plants. However, it is difficult to understand how each MAP regulates microtubule dynamics, such as growth and shortening, through mutant analyses. In vitro reconstitution analyses with individual purified MAPs and tubulin are powerful tools to reveal how each MAP regulates microtubule dynamics at the molecular level. In this review, I summarize the results of in vitro reconstitution analyses and introduce current models of how each MAP regulates microtubule dynamic instability.

Keywords: Arabidopsis; MAPs; dynamic instability; in vitro reconstitution analysis; microtubule-associated proteins; microtubules; plants; tubulin

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