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Carreras P, Law RV, Brooks N, et al. Microfluidic generation of droplet interface bilayer networks incorporating real-time size sorting in linear and non-linear configurations. Biomicrofluidics. 2014;8(5):054113doi: 10.1063/1.4897495.
Carreras, P., Law, R. V., Brooks, N., Seddon, J. M., & Ces, O. (2014). Microfluidic generation of droplet interface bilayer networks incorporating real-time size sorting in linear and non-linear configurations. Biomicrofluidics, 8(5), 054113. https://doi.org/10.1063/1.4897495
Carreras, P, et al. "Microfluidic generation of droplet interface bilayer networks incorporating real-time size sorting in linear and non-linear configurations." Biomicrofluidics vol. 8,5 (2014): 054113. doi: https://doi.org/10.1063/1.4897495
Carreras P, Law RV, Brooks N, Seddon JM, Ces O. Microfluidic generation of droplet interface bilayer networks incorporating real-time size sorting in linear and non-linear configurations. Biomicrofluidics. 2014 Oct 06;8(5):054113. doi: 10.1063/1.4897495. eCollection 2014 Sep. PMID: 25538807; PMCID: PMC4222292.
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Biomicrofluidics. 2014 Oct 06;8(5):054113. doi: 10.1063/1.4897495. eCollection 2014 Sep.

Microfluidic generation of droplet interface bilayer networks incorporating real-time size sorting in linear and non-linear configurations.

Biomicrofluidics

P Carreras, R V Law, N Brooks, J M Seddon, O Ces

Affiliations

  1. Department of Chemistry, Imperial College London , London SW7 2AZ, United Kingdom.

PMID: 25538807 PMCID: PMC4222292 DOI: 10.1063/1.4897495

Abstract

In this study, a novel droplet based microfluidic method for the generation of different sized droplet interface bilayers is reported. A microfluidic platform was designed, which allows the generation and packing of picoliter lipid coated water droplets. Droplets were generated by hydrodynamic focusing coupled with selective transport along grooves according to their size. A trapping structure at the end of the groove and a fine control of the flow pressures allowed for the droplets to be successfully trapped and aligned on demand. This technology facilitates the fine control of droplet size production as well as the generation of extended networks from a variety of lipids including 1,2-diphytanoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphocholine in linear and non-linear configurations, which is vital to the application of Droplet Interface Bilayers to biological network construction on-chip.

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