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Showing 1 to 12 of 1737 entries
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A method for reducing pressure-induced deformation in silicone microfluidics.

Biomicrofluidics

Inglis DW.
PMID: 20697573
Biomicrofluidics. 2010 Jun 17;4(2). doi: 10.1063/1.3431715.

Poly(dimethylsiloxane) or PDMS is an excellent material for replica molding, widely used in microfluidics research. Its low elastic modulus, or high deformability, assists its release from challenging molds, such as those with high feature density, high aspect ratios, and...

Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array.

Biomicrofluidics

Gel M, Kimura Y, Kurosawa O, Oana H, Kotera H, Washizu M.
PMID: 20697592
Biomicrofluidics. 2010 Jun 29;4(2). doi: 10.1063/1.3422544.

Micro-orifice based cell fusion assures high-yield fusion without compromising the cell viability. This paper examines feasibility of a dielectrophoresis (DEP) assisted cell trapping method for parallel fusion with a micro-orifice array. The goal is to create viable fusants for...

A miniaturized continuous dielectrophoretic cell sorter and its applications.

Biomicrofluidics

Valero A, Braschler T, Demierre N, Renaud P.
PMID: 20697593
Biomicrofluidics. 2010 Jun 29;4(2). doi: 10.1063/1.3430542.

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forces...

Interaction between cells in dielectrophoresis and electrorotation experiments.

Biomicrofluidics

Sancho M, Martínez G, Muñoz S, Sebastián JL, Pethig R.
PMID: 20697598
Biomicrofluidics. 2010 Jun 29;4(2). doi: 10.1063/1.3454129.

Progress in microelectrode-based technologies has facilitated the development of sophisticated methods for manipulating and separating cells, bacteria, and other bioparticles. For many of these various applications, the theoretical modeling of the electrical response of compartmentalized particles to an external...

pH controlled staining of CD4(+) and CD19(+) cells within functionalized microfluidic channel.

Biomicrofluidics

Mortato M, Blasi L, Barbarella G, Argentiere S, Gigli G.
PMID: 24191176
Biomicrofluidics. 2012 Nov 05;6(4):44107. doi: 10.1063/1.4763560. eCollection 2012.

Herein proposed is a simple system to realize hands-free labeling and simultaneous detection of two human cell lines within a microfluidic device. This system was realized by novel covalent immobilization of pH-responsive poly(methacrylic acid) microgels onto the inner glass...

Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms.

Biomicrofluidics

Geng T, Smallwood CR, Bredeweg EL, Pomraning KR, Plymale AE, Baker SE, Evans JE, Kelly RT.
PMID: 28966700
Biomicrofluidics. 2017 Sep 19;11(5):054104. doi: 10.1063/1.4986533. eCollection 2017 Sep.

Modern live-cell imaging approaches permit real-time visualization of biological processes, yet limitations exist for unicellular organism isolation, culturing, and long-term imaging that preclude fully understanding how cells sense and respond to environmental perturbations and the link between single-cell variability...

Sheathless electrokinetic particle separation in a bifurcating microchannel.

Biomicrofluidics

Li D, Lu X, Song Y, Wang J, Li D, Xuan X.
PMID: 27703590
Biomicrofluidics. 2016 Sep 16;10(5):054104. doi: 10.1063/1.4962875. eCollection 2016 Sep.

Particle separation has found practical applications in many areas from industry to academia. Current electrokinetic particle separation techniques primarily rely on dielectrophoresis, where the electric field gradients are generated by either active microelectrodes or inert micro-insulators. We develop herein...

Microfluidic assessment of swimming media for motility-based sperm selection.

Biomicrofluidics

Eamer L, Nosrati R, Vollmer M, Zini A, Sinton D.
PMID: 26339314
Biomicrofluidics. 2015 Aug 04;9(4):044113. doi: 10.1063/1.4928129. eCollection 2015 Jul.

Selection medium is important in sperm isolation for assisted reproductive technologies. Contrary to the naturally occurring human cervical mucus which has a high viscosity, most current practices for motility based sperm selection use a low viscosity medium. In this...

Microfluidics for genome-wide studies involving next generation sequencing.

Biomicrofluidics

Ma S, Murphy TW, Lu C.
PMID: 28396707
Biomicrofluidics. 2017 Mar 10;11(2):021501. doi: 10.1063/1.4978426. eCollection 2017 Mar.

Next-generation sequencing (NGS) has revolutionized how molecular biology studies are conducted. Its decreasing cost and increasing throughput permit profiling of genomic, transcriptomic, and epigenomic features for a wide range of applications. Microfluidics has been proven to be highly complementary...

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration.

Biomicrofluidics

Torino S, Iodice M, Rendina I, Coppola G, Schonbrun E.
PMID: 26634015
Biomicrofluidics. 2015 Nov 20;9(6):064107. doi: 10.1063/1.4936260. eCollection 2015 Nov.

The flow focusing is a fundamental prior step in order to sort, analyze, and detect particles or cells. The standard hydrodynamic approach requires two fluids to be injected into the microfluidic device: one containing the sample and the other...

Enhancing conjugation rate of antibodies to carboxylates: Numerical modeling of conjugation kinetics in microfluidic channels and characterization of chemical over-exposure in conventional protocols by quartz crystal microbalance.

Biomicrofluidics

Asiaei S, Smith B, Nieva P.
PMID: 26697125
Biomicrofluidics. 2015 Dec 15;9(6):064115. doi: 10.1063/1.4937929. eCollection 2015 Nov.

This research reports an improved conjugation process for immobilization of antibodies on carboxyl ended self-assembled monolayers (SAMs). The kinetics of antibody/SAM binding in microfluidic heterogeneous immunoassays has been studied through numerical simulation and experiments. Through numerical simulations, the mass...

Modelling of electrokinetic phenomena for capture of PEGylated ribonuclease A in a microdevice with insulating structures.

Biomicrofluidics

Mata-Gomez MA, Perez-Gonzalez VH, Gallo-Villanueva RC, Gonzalez-Valdez J, Rito-Palomares M, Martinez-Chapa SO.
PMID: 27375815
Biomicrofluidics. 2016 Jun 15;10(3):033106. doi: 10.1063/1.4954197. eCollection 2016 May.

Synthesis of PEGylated proteins results in a mixture of protein-polyethylene glycol (PEG) conjugates and the unreacted native protein. From a ribonuclease A (RNase A) PEGylation reaction, mono-PEGylated RNase A (mono-PEG RNase A) has proven therapeutic effects against cancer, reason...

Showing 1 to 12 of 1737 entries