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Geng T, Smallwood CR, Bredeweg EL, et al. Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms. Biomicrofluidics. 2017;11(5):054104doi: 10.1063/1.4986533.
Geng, T., Smallwood, C. R., Bredeweg, E. L., Pomraning, K. R., Plymale, A. E., Baker, S. E., Evans, J. E., & Kelly, R. T. (2017). Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms. Biomicrofluidics, 11(5), 054104. https://doi.org/10.1063/1.4986533
Geng, Tao, et al. "Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms." Biomicrofluidics vol. 11,5 (2017): 054104. doi: https://doi.org/10.1063/1.4986533
Geng T, Smallwood CR, Bredeweg EL, Pomraning KR, Plymale AE, Baker SE, Evans JE, Kelly RT. Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms. Biomicrofluidics. 2017 Sep 19;11(5):054104. doi: 10.1063/1.4986533. eCollection 2017 Sep. PMID: 28966700; PMCID: PMC5608609.
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Biomicrofluidics. 2017 Sep 19;11(5):054104. doi: 10.1063/1.4986533. eCollection 2017 Sep.

Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms.

Biomicrofluidics

Tao Geng, Chuck R Smallwood, Erin L Bredeweg, Kyle R Pomraning, Andrew E Plymale, Scott E Baker, James E Evans, Ryan T Kelly

Affiliations

  1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory Richland, Washington 99354, USA.
  2. Energy Processes and Materials Division, Pacific Northwest National Laboratory Richland, Washington 99354, USA.
  3. Biological Sciences Division, Pacific Northwest National Laboratory Richland, Washington 99354, USA.

PMID: 28966700 PMCID: PMC5608609 DOI: 10.1063/1.4986533

Abstract

Modern live-cell imaging approaches permit real-time visualization of biological processes, yet limitations exist for unicellular organism isolation, culturing, and long-term imaging that preclude fully understanding how cells sense and respond to environmental perturbations and the link between single-cell variability and whole-population dynamics. Here, we present a microfluidic platform that provides fine control over the local environment with the capacity to replace media components at any experimental time point, and provides both perfused and compartmentalized cultivation conditions depending on the valve configuration. The functionality and flexibility of the platform were validated using both bacteria and yeast having different sizes, motility, and growth media. The demonstrated ability to track the growth and dynamics of both motile and non-motile prokaryotic and eukaryotic organisms emphasizes the versatility of the devices, which should enable studies in bioenergy and environmental research.

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