Display options
Cite
Asiaei S, Smith B, Nieva P. Enhancing conjugation rate of antibodies to carboxylates: Numerical modeling of conjugation kinetics in microfluidic channels and characterization of chemical over-exposure in conventional protocols by quartz crystal microbalance. Biomicrofluidics. 2015;9(6):064115doi: 10.1063/1.4937929.
Asiaei, S., Smith, B., & Nieva, P. (2015). Enhancing conjugation rate of antibodies to carboxylates: Numerical modeling of conjugation kinetics in microfluidic channels and characterization of chemical over-exposure in conventional protocols by quartz crystal microbalance. Biomicrofluidics, 9(6), 064115. https://doi.org/10.1063/1.4937929
Asiaei, Sasan, et al. "Enhancing conjugation rate of antibodies to carboxylates: Numerical modeling of conjugation kinetics in microfluidic channels and characterization of chemical over-exposure in conventional protocols by quartz crystal microbalance." Biomicrofluidics vol. 9,6 (2015): 064115. doi: https://doi.org/10.1063/1.4937929
Asiaei S, Smith B, Nieva P. Enhancing conjugation rate of antibodies to carboxylates: Numerical modeling of conjugation kinetics in microfluidic channels and characterization of chemical over-exposure in conventional protocols by quartz crystal microbalance. Biomicrofluidics. 2015 Dec 15;9(6):064115. doi: 10.1063/1.4937929. eCollection 2015 Nov. PMID: 26697125; PMCID: PMC4684571.
Copy Download .nbib Format: NLM
Share it on

Biomicrofluidics. 2015 Dec 15;9(6):064115. doi: 10.1063/1.4937929. eCollection 2015 Nov.

Enhancing conjugation rate of antibodies to carboxylates: Numerical modeling of conjugation kinetics in microfluidic channels and characterization of chemical over-exposure in conventional protocols by quartz crystal microbalance.

Biomicrofluidics

Sasan Asiaei, Brendan Smith, Patricia Nieva

Affiliations

  1. Department of Mechanical and Mechatronics Engineering, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.

PMID: 26697125 PMCID: PMC4684571 DOI: 10.1063/1.4937929

Abstract

This research reports an improved conjugation process for immobilization of antibodies on carboxyl ended self-assembled monolayers (SAMs). The kinetics of antibody/SAM binding in microfluidic heterogeneous immunoassays has been studied through numerical simulation and experiments. Through numerical simulations, the mass transport of reacting species, namely, antibodies and crosslinking reagent, is related to the available surface concentration of carboxyl ended SAMs in a microchannel. In the bulk flow, the mass transport equation (diffusion and convection) is coupled to the surface reaction between the antibodies and SAM. The model developed is employed to study the effect of the flow rate, conjugating reagents concentration, and height of the microchannel. Dimensionless groups, such as the Damköhler number, are used to compare the reaction and fluidic phenomena present and justify the kinetic trends observed. Based on the model predictions, the conventional conjugation protocol is modified to increase the yield of conjugation reaction. A quartz crystal microbalance device is implemented to examine the resulting surface density of antibodies. As a result, an increase in surface density from 321 ng/cm(2), in the conventional protocol, to 617 ng/cm(2) in the modified protocol is observed, which is quite promising for (bio-) sensing applications.

References

  1. Anal Chem. 2003 Sep 15;75(18):4718-23 - PubMed
  2. J Am Chem Soc. 2002 Sep 4;124(35):10596-604 - PubMed
  3. Langmuir. 2010 Jan 19;26(2):809-14 - PubMed
  4. Biophys Chem. 1997 Feb 28;64(1-3):127-37 - PubMed
  5. J Chromatogr A. 2007 Oct 19;1168(1-2):170-88; discussion 169 - PubMed
  6. Sensors (Basel). 2009;9(6):4407-45 - PubMed
  7. Biotechniques. 2005 Mar;38(3):429-46 - PubMed
  8. Biosens Bioelectron. 2006 Feb 15;21(8):1424-33 - PubMed
  9. Biosens Bioelectron. 2007 Feb 15;22(7):1403-9 - PubMed
  10. Biosens Bioelectron. 2005 Jun 15;20(12):2488-503 - PubMed
  11. Biosens Bioelectron. 2009 Jan 1;24(5):1330-5 - PubMed
  12. J Org Chem. 2007 Nov 9;72(23):8863-9 - PubMed
  13. Chem Rev. 2005 Apr;105(4):1103-69 - PubMed
  14. Org Biomol Chem. 2004 Feb 7;2(3):397-401 - PubMed
  15. Front Biosci (Schol Ed). 2010 Jan 01;2:73-84 - PubMed
  16. Biosens Bioelectron. 2007 Nov 30;23(4):473-8 - PubMed
  17. Macromol Biosci. 2008 Jan 9;8(1):32-7 - PubMed
  18. Langmuir. 2004 Jul 6;20(14):5870-8 - PubMed
  19. J Immunol Methods. 1994 Feb 10;168(2):227-34 - PubMed
  20. Biophys J. 1998 Aug;75(2):583-94 - PubMed
  21. Biomed Microdevices. 2005 Jun;7(2):99-110 - PubMed
  22. J Zhejiang Univ Sci B. 2008 Feb;9(2):121-31 - PubMed

Publication Types