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Torino S, Iodice M, Rendina I, et al. Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration. Biomicrofluidics. 2015;9(6):064107doi: 10.1063/1.4936260.
Torino, S., Iodice, M., Rendina, I., Coppola, G., & Schonbrun, E. (2015). Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration. Biomicrofluidics, 9(6), 064107. https://doi.org/10.1063/1.4936260
Torino, S, et al. "Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration." Biomicrofluidics vol. 9,6 (2015): 064107. doi: https://doi.org/10.1063/1.4936260
Torino S, Iodice M, Rendina I, Coppola G, Schonbrun E. Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration. Biomicrofluidics. 2015 Nov 20;9(6):064107. doi: 10.1063/1.4936260. eCollection 2015 Nov. PMID: 26634015; PMCID: PMC4654736.
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Biomicrofluidics. 2015 Nov 20;9(6):064107. doi: 10.1063/1.4936260. eCollection 2015 Nov.

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration.

Biomicrofluidics

S Torino, M Iodice, I Rendina, G Coppola, E Schonbrun

Affiliations

  1. Institute for Microelectronics and Microsystems , National Research Council, Naples, Italy.
  2. Rowland Institute at Harvard, Harvard University , 100 E. Land Blvd., Cambridge, Massachusetts 02142, USA.

PMID: 26634015 PMCID: PMC4654736 DOI: 10.1063/1.4936260

Abstract

The flow focusing is a fundamental prior step in order to sort, analyze, and detect particles or cells. The standard hydrodynamic approach requires two fluids to be injected into the microfluidic device: one containing the sample and the other one, called the sheath fluid, allows squeezing the sample fluid into a narrow stream. The major drawback of this approach is the high complexity of the layout for microfluidic devices when parallel streams are required. In this work, we present a novel parallelized microfluidic device that enables hydrodynamic focusing in each microchannel using a single feed flow. At each of the parallel channels, a cross-filter region is present that allows removing fluid from the sample fluid. This fluid is used to create local sheath fluids that hydrodynamically pinch the sample fluid. The great advantage of the proposed device is that, since only one inlet is needed, multiple parallel micro-channels can be easily introduced into the design. In the paper, the design method is described and the numerical simulations performed to define the optimal design are summarized. Moreover, the operational functionality of devices tested by using both polystyrene beads and Acute Lymphoid Leukemia cells are shown.

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