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Wewer Albrechtsen NJ, Bak MJ, Hartmann B, et al. Stability of glucagon-like peptide 1 and glucagon in human plasma. Endocr Connect. 2015;4(1):50-7doi: 10.1530/EC-14-0126.
Wewer Albrechtsen, N. J., Bak, M. J., Hartmann, B., Christensen, L. W., Kuhre, R. E., Deacon, C. F., & Holst, J. J. (2015). Stability of glucagon-like peptide 1 and glucagon in human plasma. Endocrine connections, 4(1), 50-7. https://doi.org/10.1530/EC-14-0126
Wewer Albrechtsen, Nicolai J, et al. "Stability of glucagon-like peptide 1 and glucagon in human plasma." Endocrine connections vol. 4,1 (2015): 50-7. doi: https://doi.org/10.1530/EC-14-0126
Wewer Albrechtsen NJ, Bak MJ, Hartmann B, Christensen LW, Kuhre RE, Deacon CF, Holst JJ. Stability of glucagon-like peptide 1 and glucagon in human plasma. Endocr Connect. 2015 Mar;4(1):50-7. doi: 10.1530/EC-14-0126. Epub 2015 Jan 16. PMID: 25596009; PMCID: PMC4317691.
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Endocr Connect. 2015 Mar;4(1):50-7. doi: 10.1530/EC-14-0126. Epub 2015 Jan 16.

Stability of glucagon-like peptide 1 and glucagon in human plasma.

Endocrine connections

Nicolai J Wewer Albrechtsen, Monika J Bak, Bolette Hartmann, Louise Wulff Christensen, Rune E Kuhre, Carolyn F Deacon, Jens J Holst

Affiliations

  1. NNF Center for Basic Metabolic Research and Department of Biomedical SciencesDepartment of ScienceFaculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK-2200 Copenhagen N, Denmark.
  2. NNF Center for Basic Metabolic Research and Department of Biomedical SciencesDepartment of ScienceFaculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK-2200 Copenhagen N, Denmark NNF Center for Basic Metabolic Research and Department of Biomedical SciencesDepartment of ScienceFaculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK-2200 Copenhagen N, Denmark.
  3. NNF Center for Basic Metabolic Research and Department of Biomedical SciencesDepartment of ScienceFaculty of Health Science, University of Copenhagen, Blegdamsvej 3B, 12.2, DK-2200 Copenhagen N, Denmark [email protected].

PMID: 25596009 PMCID: PMC4317691 DOI: 10.1530/EC-14-0126

Abstract

To investigate the stability of glucagon-like peptide 1 (GLP-1) and glucagon in plasma under short- and long-term storage conditions. Pooled human plasma (n=20), to which a dipeptidyl peptidase 4 (DPP4) inhibitor and aprotinin were added, was spiked with synthetic GLP-1 (intact, 7-36NH2 as well as the primary metabolite, GLP-1 9-36NH2) or glucagon. Peptide recoveries were measured in samples kept for 1 and 3 h at room temperature or on ice, treated with various enzyme inhibitors, after up to three thawing-refreezing cycles, and after storage at -20 and -80 °C for up to 1 year. Recoveries were unaffected by freezing cycles or if plasma was stored on ice for up to 3 h, but were impaired when samples stood at RT for more than 1 h. Recovery of intact GLP-1 increased by addition of a DPP4 inhibitor (no ice), but was not further improved by neutral endopeptidase 24.11 inhibitor or an inhibitor cocktail. GLP-1, but not glucagon, was stable for at least 1 year. Surprisingly, the recovery of glucagon was reduced by almost 50% by freezing compared with immediate analysis, regardless of storage time. Plasma handling procedures can significantly influence results of subsequent hormone analysis. Our data support addition of DPP4 inhibitor for GLP-1 measurement as well as cooling on ice of both GLP-1 and glucagon. Freeze-thaw cycles did not significantly affect stability of GLP-1 or glucagon. Long-term storage may affect glucagon levels regardless of storage temperature and results should be interpreted with caution.

© 2015 The authors.

Keywords: degradation; glucagon; glucagon-like peptide-1; samples handling; storage

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