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Joo M, Kim HS, Kwon TH, et al. Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats. Korean J Physiol Pharmacol. 2014;19(1):43-50doi: 10.4196/kjpp.2015.19.1.43.
Joo, M., Kim, H. S., Kwon, T. H., Palikhe, A., Zaw, T. S., Jeong, J. H., & Sohn, U. D. (2015). Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 19(1), 43-50. https://doi.org/10.4196/kjpp.2015.19.1.43
Joo, Minjae, et al. "Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats." The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology vol. 19,1 (2015): 43-50. doi: https://doi.org/10.4196/kjpp.2015.19.1.43
Joo M, Kim HS, Kwon TH, Palikhe A, Zaw TS, Jeong JH, Sohn UD. Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats. Korean J Physiol Pharmacol. 2015 Jan;19(1):43-50. doi: 10.4196/kjpp.2015.19.1.43. Epub 2014 Dec 31. PMID: 25605996; PMCID: PMC4297761.
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Korean J Physiol Pharmacol. 2015 Jan;19(1):43-50. doi: 10.4196/kjpp.2015.19.1.43. Epub 2014 Dec 31.

Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Minjae Joo, Han Sang Kim, Tae Hoon Kwon, Alisha Palikhe, Tin Sandar Zaw, Ji Hoon Jeong, Uy Dong Sohn

Affiliations

  1. Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
  2. Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.

PMID: 25605996 PMCID: PMC4297761 DOI: 10.4196/kjpp.2015.19.1.43

Abstract

It has been shown that the extracts including eupatilin and quercetin-3-β-D-glucuronopyranoside had mucoprotective effects on the esophagus and stomach through their antioxidant activities. This study was designed to investigate the anti-inflammatory effect of these flavonoid compounds in an animal model of inflammatory bowel disease induced by 2,4,6-trinitrobenzene sulfonic acid. Experimental colitis was induced by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid. Extracts including eupatilin or quercetin-3-β-D-glucuronopyranoside were orally administered to animals 48, 24, and 1 h prior to the induction of colitis and then again 24 h later. The animals were sacrificed 48 h after by 2,4,6-trinitrobenzene sulfonic acid treatment and the macroscopic appearance of the colonic lesions was scored in a blinded manner on a scale of 1 to 10. The inflammatory response to colitis induction was assessed by measuring myeloperoxidase activity, nitric oxide production, tumor necrosis factor-α expression, total glutathione levels, and malondialdehyde concentrations in the colon. The results indicated that extracts including eupatilin and extracts including quercetin-3-β-D-glucuronopyranoside dose-dependently improved the morphology of the lesions induced by 2,4,6-trinitrobenzene sulfonic acid and reduced the ulcer index accordingly. In addition, rats receiving extracts including eupatilin and extracts including quercetin-3-β-D-glucuronopyranoside showed significantly decreased levels of mucosal myeloperoxidase activity, nitric oxide production, tumor necrosis factor-α expression, and malondialdehyde levels, and increased total glutathione levels. Extracts including eupatilin and extracts including quercetin-3-β-D-glucuronopyranoside ameliorated the inflammatory response and colonic injury in acute colitis by decreasing oxidative stress and neutrophil activation. Extracts including eupatilin and extracts including quercetin-3-β-D-glucuronopyranoside may inhibit acute colitis.

Keywords: Colitis; Flavonoids; Inflammation; Quercetin; Reactive oxygen species

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