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Wettstein P, Priest C, Al-Bataineh SA, et al. Surface protein gradients generated in sealed microchannels using spatially varying helium microplasma. Biomicrofluidics. 2015;9(1):014124doi: 10.1063/1.4913367.
Wettstein, P., Priest, C., Al-Bataineh, S. A., Short, R. D., Bryant, P. M., Bradley, J. W., Low, S. P., Parkinson, L., & Szili, E. J. (2015). Surface protein gradients generated in sealed microchannels using spatially varying helium microplasma. Biomicrofluidics, 9(1), 014124. https://doi.org/10.1063/1.4913367
Wettstein, Pascal, et al. "Surface protein gradients generated in sealed microchannels using spatially varying helium microplasma." Biomicrofluidics vol. 9,1 (2015): 014124. doi: https://doi.org/10.1063/1.4913367
Wettstein P, Priest C, Al-Bataineh SA, Short RD, Bryant PM, Bradley JW, Low SP, Parkinson L, Szili EJ. Surface protein gradients generated in sealed microchannels using spatially varying helium microplasma. Biomicrofluidics. 2015 Feb 20;9(1):014124. doi: 10.1063/1.4913367. eCollection 2015 Jan. PMID: 25759757; PMCID: PMC4336250.
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Biomicrofluidics. 2015 Feb 20;9(1):014124. doi: 10.1063/1.4913367. eCollection 2015 Jan.

Surface protein gradients generated in sealed microchannels using spatially varying helium microplasma.

Biomicrofluidics

Pascal Wettstein, Craig Priest, Sameer A Al-Bataineh, Robert D Short, Paul M Bryant, James W Bradley, Suet P Low, Luke Parkinson, Endre J Szili

Affiliations

  1. Mawson Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
  2. Ian Wark Research Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
  3. Department of Electrical Engineering and Electronics, The University of Liverpool , Brownlow Hill, Liverpool L69 3GJ, United Kingdom.

PMID: 25759757 PMCID: PMC4336250 DOI: 10.1063/1.4913367

Abstract

Spatially varied surface treatment of a fluorescently labeled Bovine Serum Albumin (BSA) protein, on the walls of a closed (sealed) microchannel is achieved via a well-defined gradient in plasma intensity. The microchips comprised a microchannel positioned in-between two microelectrodes (embedded in the chip) with a variable electrode separation along the length of the channel. The channel and electrodes were 50 μm and 100 μm wide, respectively, 50 μm deep, and adjacent to the channel for a length of 18 mm. The electrode separation distance was varied linearly from 50 μm at one end of the channel to a maximum distance of 150, 300, 500, or 1000 μm to generate a gradient in helium plasma intensity. Plasma ignition was achieved at a helium flow rate of 2.5 ml/min, 8.5 kVpk-pk, and 10 kHz. It is shown that the plasma intensity decreases with increasing electrode separation and is directly related to the residual amount of BSA left after the treatment. The plasma intensity and surface protein gradient, for the different electrode gradients studied, collapse onto master curves when plotted against electrode separation. This precise spatial control is expected to enable the surface protein gradient to be tuned for a range of applications, including high-throughput screening and cell-biomolecule-biomaterial interactions.

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