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Matsunaga A, Takami M, Irié T, et al. Microscopic study on resorption of β-tricalcium phosphate materials by osteoclasts. Cytotechnology. 2015;67(4):727-32doi: 10.1007/s10616-015-9854-0.
Matsunaga, A., Takami, M., Irié, T., Mishima, K., Inagaki, K., & Kamijo, R. (2015). Microscopic study on resorption of β-tricalcium phosphate materials by osteoclasts. Cytotechnology, 67(4), 727-32. https://doi.org/10.1007/s10616-015-9854-0
Matsunaga, Akihiro, et al. "Microscopic study on resorption of β-tricalcium phosphate materials by osteoclasts." Cytotechnology vol. 67,4 (2015): 727-32. doi: https://doi.org/10.1007/s10616-015-9854-0
Matsunaga A, Takami M, Irié T, Mishima K, Inagaki K, Kamijo R. Microscopic study on resorption of β-tricalcium phosphate materials by osteoclasts. Cytotechnology. 2015 Aug;67(4):727-32. doi: 10.1007/s10616-015-9854-0. Epub 2015 Feb 12. PMID: 25672942; PMCID: PMC4474988.
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Cytotechnology. 2015 Aug;67(4):727-32. doi: 10.1007/s10616-015-9854-0. Epub 2015 Feb 12.

Microscopic study on resorption of β-tricalcium phosphate materials by osteoclasts.

Cytotechnology

Akihiro Matsunaga, Masamichi Takami, Tarou Irié, Kenji Mishima, Katsunori Inagaki, Ryutaro Kamijo

Affiliations

  1. Department of Biochemistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo, 142-8555, Japan.

PMID: 25672942 PMCID: PMC4474988 DOI: 10.1007/s10616-015-9854-0

Abstract

Sintered compounds prepared with β-tricalcium phosphate (β-TCP) are commonly used as biocompatible materials for bone regenerative medicine. Although implanted β-TCP is gradually replaced with new bone after resorption by osteoclasts, exactly how osteoclasts resorb β-TCP is not well understood. To elucidate this mechanism, we analyzed the structure of β-TCP discs on which mouse mature osteoclasts were cultured using scanning electron microscopy. We found that β-TCP was resorbed by mature osteoclasts on one side of each disc, as evidenced by the formation of multiple spine-like crystals at the exposed areas. Because osteoclasts secrete acid to resorb bone minerals, we mimicked this acidification by dipping β-TCP slices into HCl solution (pH 2.0). However, no spine-like crystals appeared even though the size of each β-TCP particle was reduced. On dentin slices, osteoclasts formed clear actin rings, which are cytoskeletal structures characteristic of bone-resorbing osteoclasts. No clear actin rings were observed in osteoclasts cultured on β-TCP slices, although small actin dots were observed. Analysis by transmission electron microscopy showed that osteoclasts attached to β-TCP particles. These results suggest that osteoclasts resorb β-TCP particles independently of clear actin ring formation.

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