J Int Soc Sports Nutr. 2015 Feb 06;12:7. doi: 10.1186/s12970-015-0067-x. eCollection 2015.
Glutathione supplementation suppresses muscle fatigue induced by prolonged exercise via improved aerobic metabolism.
Journal of the International Society of Sports Nutrition
Wataru Aoi, Yumi Ogaya, Maki Takami, Toru Konishi, Yusuke Sauchi, Eun Young Park, Sayori Wada, Kenji Sato, Akane Higashi
Affiliations
Affiliations
- Laboratory of Health Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho Shimogamo, Sakyo-ku, Kyoto 606-8522 Japan.
- KOHJIN Life Sciences Company, Ltd., Tokyo, Japan.
- Laboratory of Food Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
PMID: 25685110
PMCID: PMC4328900 DOI: 10.1186/s12970-015-0067-x
Abstract
BACKGROUNDS: Glutathione is an endogenous redox couple in animal cells and plays important roles in antioxidant defense and detoxification, although it is unknown if oral glutathione supplementation affects exercise-induced physiological changes. The present study investigated the effect of glutathione intake on exercise-induced muscle metabolism and fatigue in mice and humans.
METHODS: ICR mice were divided into 4 groups: sedentary control, sedentary supplemented with glutathione (2.0%, 5 μL/g body weight), exercise control, and exercise supplemented with glutathione. After 2 weeks, the exercise groups ran on a treadmill at 25 m/min for 30 min. Immediately post-exercise, intermuscular pH was measured, and hind limb muscle and blood samples were collected to measure biochemical parameters. In a double-blind, cross-over study, 8 healthy men (35.9 ± 2.0 y) were administered either glutathione (1 g/d) or placebo for 2 weeks. Then, they exercised on a cycle ergometer at 40% maximal heart rate for 60 min. Psychological state and blood biochemical parameters were examined after exercise.
RESULTS: In the mouse experiment, post-exercise plasma non-esterified fatty acids were significantly lower in the exercise supplemented with glutathione group (820 ± 44 mEq/L) compared with the exercise control group (1152 ± 61 mEq/L). Intermuscular pH decreased with exercise (7.17 ± 0.01); however, this reduction was prevented by glutathione supplementation (7.23 ± 0.02). The peroxisome proliferator-activated receptor-γ coactivator-1α protein and mitochondrial DNA levels were significantly higher in the sedentary supplemented with glutathione group compared with the sedentary control group (25% and 53% higher, respectively). In the human study, the elevation of blood lactate was suppressed by glutathione intake (placebo, 3.4 ± 1.1 mM; glutathione, 2.9 ± 0.6 mM). Fatigue-related psychological factors were significantly decreased in the glutathione trial compared with the placebo trial.
CONCLUSIONS: These results suggest that glutathione supplementation improved lipid metabolism and acidification in skeletal muscles during exercise, leading to less muscle fatigue.
Keywords: Dkeletal muscle; Glutathione; Lipid metabolism; PGC-1α; Running exercise
References
- Biochem Biophys Res Commun. 2006 Feb 3;340(1):291-5 - PubMed
- Med Sci Sports Exerc. 1986 Jun;18(3):360-8 - PubMed
- Biochem Biophys Res Commun. 1986 Sep 30;139(3):926-31 - PubMed
- Can J Physiol Pharmacol. 1985 May;63(5):403-16 - PubMed
- Acta Physiol (Oxf). 2008 Dec;194(4):283-91 - PubMed
- Pflugers Arch. 2010 Jun;460(1):153-62 - PubMed
- Nutrients. 2012 Oct 09;4(10):1399-440 - PubMed
- J Appl Physiol (1985). 2008 May;104(5):1304-12 - PubMed
- Cell Metab. 2010 Dec 1;12(6):633-42 - PubMed
- J Agric Food Chem. 2014 Jul 2;62(26):6183-9 - PubMed
- Acta Physiol Scand. 1973 Jul;88(3):350-8 - PubMed
- J Clin Invest. 2006 Mar;116(3):615-22 - PubMed
- Biochem Biophys Res Commun. 2002 Aug 16;296(2):350-4 - PubMed
- J Biol Chem. 1994 Apr 1;269(13):9397-400 - PubMed
- Am J Clin Nutr. 2008 Jan;87(1):142-9 - PubMed
- Cancer Treat Rev. 1990 Sep;17(2-3):203-8 - PubMed
- PLoS One. 2011;6(12 ):e28290 - PubMed
- J Physiol. 1995 Oct 1;488 ( Pt 1):219-29 - PubMed
- Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20405-10 - PubMed
- Cell. 1999 Jul 9;98 (1):115-24 - PubMed
- J Appl Physiol (1985). 1992 Feb;72(2):549-54 - PubMed
- Eur J Clin Invest. 1991 Oct;21(5):534-41 - PubMed
- J Physiol. 1999 Jun 15;517 ( Pt 3):633-42 - PubMed
- Clin Chem. 1995 Oct;41(10):1522-5 - PubMed
- Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8466-71 - PubMed
- J Physiol Biochem. 2001 Mar;57(1):9-14 - PubMed
- J Agric Food Chem. 2014 Oct 1;62(39):9499-506 - PubMed
- FEBS Lett. 1985 Jun 17;185(2):262-6 - PubMed
- Arch Physiol Biochem. 2007 Oct-Dec;113(4-5):234-58 - PubMed
- Proc Natl Acad Sci U S A. 2009 May 26;106(21):8665-70 - PubMed
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