Display options
Cite
Lu W, Zheng J, Pan XD, et al. A new simplified volume-loaded heterotopic rabbit heart transplant model with improved techniques and a standard operating procedure. J Thorac Dis. 2015;7(4):653-61doi: 10.3978/j.issn.2072-1439.2015.03.05.
Lu, W., Zheng, J., Pan, X. D., Li, B., Zhang, J. W., Wang, L. F., & Sun, L. Z. (2015). A new simplified volume-loaded heterotopic rabbit heart transplant model with improved techniques and a standard operating procedure. Journal of thoracic disease, 7(4), 653-61. https://doi.org/10.3978/j.issn.2072-1439.2015.03.05
Lu, Wei, et al. "A new simplified volume-loaded heterotopic rabbit heart transplant model with improved techniques and a standard operating procedure." Journal of thoracic disease vol. 7,4 (2015): 653-61. doi: https://doi.org/10.3978/j.issn.2072-1439.2015.03.05
Lu W, Zheng J, Pan XD, Li B, Zhang JW, Wang LF, Sun LZ. A new simplified volume-loaded heterotopic rabbit heart transplant model with improved techniques and a standard operating procedure. J Thorac Dis. 2015 Apr;7(4):653-61. doi: 10.3978/j.issn.2072-1439.2015.03.05. PMID: 25973231; PMCID: PMC4419328.
Copy Download .nbib Format: NLM
Share it on

J Thorac Dis. 2015 Apr;7(4):653-61. doi: 10.3978/j.issn.2072-1439.2015.03.05.

A new simplified volume-loaded heterotopic rabbit heart transplant model with improved techniques and a standard operating procedure.

Journal of thoracic disease

Wei Lu, Jun Zheng, Xu-Dong Pan, Bing Li, Jin-Wei Zhang, Long-Fei Wang, Li-Zhong Sun

Affiliations

  1. 1 Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China ; 2 Department of Pediatric Heart center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.

PMID: 25973231 PMCID: PMC4419328 DOI: 10.3978/j.issn.2072-1439.2015.03.05

Abstract

BACKGROUND: The classic non-working (NW) heterotopic heart transplant (HTX) model in rodents had been widely used for researches related to immunology, graft rejection, evaluation of immunosuppressive therapies and organ preservation. But unloaded models are considered not suitable for some researches. Accordingly, We have constructed a volume-loaded (VL) model by a new and simple technique.

METHODS: Thirty male New Zealand White rabbits were randomly divided into two groups, group NW with 14 rabbits and group VL with 16 rabbits, which served as donors and recipients. We created a large and nonrestrictive shunt to provide left heart a sufficient preload. The donor superior vena cave and ascending aorta (AO) were anastomosed to the recipient abdominal aorta (AAO) and inferior vena cava (IVC), respectively.

RESULTS: No animals suffered from paralysis, pneumonia and lethal bleeding. Recipients' mortality and morbidity were 6.7% (1/15) and 13.3% (2/15), respectively. The cold ischemia time in group VL is slight longer than that in group NW. The maximal aortic velocity (MAV) of donor heart was approximately equivalent to half that of native heart in group VL. Moreover, the similar result was achieved in the parameter of late diastolic mitral inflow velocity between donor heart and native heart in group VL. The echocardiography (ECHO) showed a bidirectional flow in donor SVC of VL model, inflow during diastole and outflow during systole. PET-CT imaging showed the standard uptake value (SUV) of allograft was equal to that of native heart in both groups on the postoperative day 3.

CONCLUSIONS: We have developed a new VL model in rabbits, which imitates a native heart hemodynamically while only requiring a minor additional procedure. Surgical technique is simple compared with currently used HTX models. We also developed a standard operating procedure that significantly improved graft and recipient survival rate. This study may be useful for investigations in transplantation in which a working model is required.

Keywords: Heterotopic heart transplantation (HTX); standard operating procedure; volume-loaded model (VL model)

References

  1. Bioconjug Chem. 2010 May 19;21(5):884-91 - PubMed
  2. Transplantation. 1965 May;3:432-45 - PubMed
  3. Arch Pathol. 1972 Mar;93(3):240-5 - PubMed
  4. Transplant Proc. 2013 Jul-Aug;45(6):2522-6 - PubMed
  5. Arch Surg. 1964 Oct;89:645-52 - PubMed
  6. Comp Med. 2012 Apr;62(2):116-23 - PubMed
  7. J Surg Res. 2007 May 15;139(2):157-63 - PubMed
  8. Transplantation. 1990 Apr;49(4):835-7 - PubMed
  9. Comp Med. 2014 Aug;64(4):293-9 - PubMed
  10. J Heart Lung Transplant. 2003 Aug;22(8):937-45 - PubMed
  11. Acta Pathol Microbiol Scand A. 1971;79(4):366-72 - PubMed
  12. Transplantation. 1985 Nov;40(5):567-9 - PubMed
  13. Am J Transl Res. 2013 Aug 15;5(5):521-9 - PubMed
  14. Circ Res. 1991 Apr;68(4):1100-7 - PubMed
  15. Exp Clin Transplant. 2011 Apr;9(2):128-33 - PubMed
  16. Transplant Proc. 1994 Aug;26(4):2320-2 - PubMed
  17. J Thorac Cardiovasc Surg. 2011 Jun;141(6):1522-8 - PubMed
  18. Transplantation. 1997 Jul 27;64(2):215-22 - PubMed
  19. J Heart Lung Transplant. 1992 Nov-Dec;11(6):1147-50 - PubMed
  20. J Heart Transplant. 1986 Nov-Dec;5(6):465-70 - PubMed
  21. J Thorac Cardiovasc Surg. 1969 Feb;57(2):225-9 - PubMed
  22. Transplantation. 1969 Mar;7(3):176-82 - PubMed
  23. J Nucl Med. 2006 Aug;47(8):1367-74 - PubMed
  24. J Nucl Cardiol. 2010 Aug;17 (4):631-6 - PubMed
  25. Transpl Immunol. 2011 Jul;25(1):82-7 - PubMed
  26. Res Vet Sci. 2014 Oct;97(2):376-81 - PubMed
  27. Ann Thorac Surg. 1993 Nov;56(5):1074-7 - PubMed

Publication Types