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Kim H, Cheang UK, Kim D, et al. Hydrodynamics of a self-actuated bacterial carpet using microscale particle image velocimetry. Biomicrofluidics. 2015;9(2):024121doi: 10.1063/1.4918978.
Kim, H., Cheang, U. K., Kim, D., Ali, J., & Kim, M. J. (2015). Hydrodynamics of a self-actuated bacterial carpet using microscale particle image velocimetry. Biomicrofluidics, 9(2), 024121. https://doi.org/10.1063/1.4918978
Kim, Hoyeon, et al. "Hydrodynamics of a self-actuated bacterial carpet using microscale particle image velocimetry." Biomicrofluidics vol. 9,2 (2015): 024121. doi: https://doi.org/10.1063/1.4918978
Kim H, Cheang UK, Kim D, Ali J, Kim MJ. Hydrodynamics of a self-actuated bacterial carpet using microscale particle image velocimetry. Biomicrofluidics. 2015 Apr 23;9(2):024121. doi: 10.1063/1.4918978. eCollection 2015 Mar. PMID: 26015833; PMCID: PMC4409625.
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Biomicrofluidics. 2015 Apr 23;9(2):024121. doi: 10.1063/1.4918978. eCollection 2015 Mar.

Hydrodynamics of a self-actuated bacterial carpet using microscale particle image velocimetry.

Biomicrofluidics

Hoyeon Kim, U Kei Cheang, Dalhyung Kim, Jamel Ali, Min Jun Kim

Affiliations

  1. Department of Mechanical Engineering and Mechanics, Drexel University , Philadelphia, Pennsylvania 19104, USA.

PMID: 26015833 PMCID: PMC4409625 DOI: 10.1063/1.4918978

Abstract

Microorganisms can effectively generate propulsive force at the microscale where viscous forces overwhelmingly dominate inertia forces; bacteria achieve this task through flagellar motion. When swarming bacteria, cultured on agar plates, are blotted onto the surface of a microfabricated structure, a monolayer of bacteria forms what is termed a "bacterial carpet," which generates strong flows due to the combined motion of their freely rotating flagella. Furthermore, when the bacterial carpet coated microstructure is released into a low Reynolds number fluidic environment, the propulsive force of the bacterial carpet is able to give the microstructure motility. In our previous investigations, we demonstrated motion control of these bacteria powered microbiorobots (MBRs). Without any external stimuli, MBRs display natural rotational and translational movements on their own; this MBR self-actuation is due to the coordination of flagella. Here, we investigate the flow fields generated by bacterial carpets, and compare this flow to the flow fields observed in the bulk fluid at a series of locations above the bacterial carpet. Using microscale particle image velocimetry, we characterize the flow fields generated from the bacterial carpets of MBRs in an effort to understand their propulsive flow, as well as the resulting pattern of flagella driven self-actuated motion. Comparing the velocities between the bacterial carpets on fixed and untethered MBRs, it was found that flow velocities near the surface of the microstructure were strongest, and at distances far above, the surface flow velocities were much smaller.

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