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Satpathy S, Batabyal S, Dhakal KR, et al. Broad spectral excitation of opsin for enhanced stimulation of cells. Opt Lett. 2015;40(11):2465-8doi: 10.1364/OL.40.002465.
Satpathy, S., Batabyal, S., Dhakal, K. R., Lin, J., Kim, Y. T., & Mohanty, S. K. (2015). Broad spectral excitation of opsin for enhanced stimulation of cells. Optics letters, 40(11), 2465-8. https://doi.org/10.1364/OL.40.002465
Satpathy, Sarmishtha, et al. "Broad spectral excitation of opsin for enhanced stimulation of cells." Optics letters vol. 40,11 (2015): 2465-8. doi: https://doi.org/10.1364/OL.40.002465
Satpathy S, Batabyal S, Dhakal KR, Lin J, Kim YT, Mohanty SK. Broad spectral excitation of opsin for enhanced stimulation of cells. Opt Lett. 2015 Jun 01;40(11):2465-8. doi: 10.1364/OL.40.002465. PMID: 26030533.
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Opt Lett. 2015 Jun 01;40(11):2465-8. doi: 10.1364/OL.40.002465.

Broad spectral excitation of opsin for enhanced stimulation of cells.

Optics letters

Sarmishtha Satpathy, Subrata Batabyal, Kamal R Dhakal, John Lin, Young-tae Kim, Samarendra K Mohanty

PMID: 26030533 DOI: 10.1364/OL.40.002465

Abstract

Optical stimulation of cells expressing light-sensitive proteins (opsins) has allowed targeted activation with cellular specificity. However, since narrow-band light has been used for excitation of these optogenetic probes, only active stimulation strategies are being attempted for clinical applications such as restoration of vision. Here, we report use of broad spectral excitation (white light) for optogenetic stimulation of opsin-sensitized cells. We found that ReaChR is optimally excited with white light offering significantly higher photocurrents compared to spectrally filtered narrow-band light stimulation. Our findings open up the possibility of passive stimulation strategy by use of natural sunlight for retinal stimulation, which could have benefits for ambient light stimulated vision restoration.

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