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Hwang DW, Park KM, Shim HK, et al. In vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels. EJNMMI Res. 2014;4(1):61doi: 10.1186/s13550-014-0061-3.
Hwang, D. W., Park, K. M., Shim, H. K., Jin, Y., Oh, H. J., Oh, S. W., Lee, S., Youn, H., Joung, Y. K., Lee, H. J., Kim, S. U., Park, K. D., & Lee, D. S. (2014). In vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels. EJNMMI research, 4(1), 61. https://doi.org/10.1186/s13550-014-0061-3
Hwang, Do Won, et al. "In vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels." EJNMMI research vol. 4,1 (2014): 61. doi: https://doi.org/10.1186/s13550-014-0061-3
Hwang DW, Park KM, Shim HK, Jin Y, Oh HJ, Oh SW, Lee S, Youn H, Joung YK, Lee HJ, Kim SU, Park KD, Lee DS. In vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels. EJNMMI Res. 2014 Dec;4(1):61. doi: 10.1186/s13550-014-0061-3. Epub 2014 Nov 12. PMID: 26116122; PMCID: PMC4452629.
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EJNMMI Res. 2014 Dec;4(1):61. doi: 10.1186/s13550-014-0061-3. Epub 2014 Nov 12.

In vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels.

EJNMMI research

Do Won Hwang, Kyung Min Park, Hye-Kyung Shim, Yeona Jin, Hyun Jeong Oh, So Won Oh, Song Lee, Hyewon Youn, Yoon Ki Joung, Hong J Lee, Seung U Kim, Ki Dong Park, Dong Soo Lee

Affiliations

  1. Department of Nuclear Medicine, Seoul National University College of Medicine, 28 Yongon-Dong, Jongno-Gu, Seoul, 110-744, Republic of Korea, [email protected].

PMID: 26116122 PMCID: PMC4452629 DOI: 10.1186/s13550-014-0061-3

Abstract

BACKGROUND: Three-dimensional (3D) hydrogel-based stem cell therapies contribute to enhanced therapeutic efficacy in treating diseases, and determining the optimal mechanical strength of the hydrogel in vivo is important for therapeutic success. We evaluated the proliferation of human neural stem cells incorporated within in situ-forming hydrogels and compared the effect of hydrogels with different elastic properties in cell/hydrogel-xenografted mice.

METHODS: The gelatin-polyethylene glycol-tyramine (GPT) hydrogel was fabricated through enzyme-mediated cross-linking reaction using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2).

RESULTS: The F3-effluc encapsulated within a soft 1,800 pascal (Pa) hydrogel and stiff 5,800 Pa hydrogel proliferated vigorously in a 24-well plate until day 8. In vitro and in vivo kinetics of luciferase activity showed a slow time-to-peak after D-luciferin administration in the stiff hydrogel. When in vivo proliferation of F3-effluc was observed up to day 21 in both the hydrogel group and cell-only group, F3-effluc within the soft hydrogel proliferated more vigorously, compared to the cells within the stiff hydrogel. Ki-67-specific immunostaining revealed highly proliferative F3-effluc with compactly distributed cell population inside the 1,800 Pa or 5,800 Pa hydrogel.

CONCLUSIONS: We examined the in vivo effectiveness of different elastic types of hydrogels encapsulating viable neural stem cells by successfully monitoring the proliferation of implanted stem cells incorporated within a 3D hydrogel scaffold.

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