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Xiong J, Jiang HP, Peng CY, et al. DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry. Clin Epigenetics. 2015;7:72doi: 10.1186/s13148-015-0109-x.
Xiong, J., Jiang, H. P., Peng, C. Y., Deng, Q. Y., Lan, M. D., Zeng, H., Zheng, F., Feng, Y. Q., & Yuan, B. F. (2015). DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry. Clinical epigenetics, 772. https://doi.org/10.1186/s13148-015-0109-x
Xiong, Jun, et al. "DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry." Clinical epigenetics vol. 7 (2015): 72. doi: https://doi.org/10.1186/s13148-015-0109-x
Xiong J, Jiang HP, Peng CY, Deng QY, Lan MD, Zeng H, Zheng F, Feng YQ, Yuan BF. DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry. Clin Epigenetics. 2015 Jul 23;7:72. doi: 10.1186/s13148-015-0109-x. eCollection 2015. PMID: 26207150; PMCID: PMC4511989.
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Clin Epigenetics. 2015 Jul 23;7:72. doi: 10.1186/s13148-015-0109-x. eCollection 2015.

DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry.

Clinical epigenetics

Jun Xiong, Han-Peng Jiang, Chun-Yan Peng, Qian-Yun Deng, Meng-Dan Lan, Huan Zeng, Fang Zheng, Yu-Qi Feng, Bi-Feng Yuan

Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of education), Department of Chemistry, Wuhan University, Wuhan, 430072 China.
  2. Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, 430071 China.

PMID: 26207150 PMCID: PMC4511989 DOI: 10.1186/s13148-015-0109-x

Abstract

BACKGROUND: Aging is a complex phenomenon and characterized by a progressive decline in physiology and function of adult tissues. However, it hasn't been well established of the correlation between aging and global DNA methylation and hydroxymethylation that regulate the growth and development of higher organisms.

RESULTS: We developed an on-line trapping/capillary hydrophilic-interaction liquid chromatography/electrospray ionization-mass spectrometry method for ultra-sensitive and simultaneous quantification of 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) in genomic DNA from human blood. Limits of detection for 5-mC and 5-hmC were 0.04 and 0.13 fmol, respectively. The imprecision and recovery of the method were determined with the relative standard deviations (RSDs) and relative errors being <11.2 and 14.0 %, respectively. We analyzed the contents of 5-mC and 5-hmC in genomic DNA of blood from 238 healthy people aged from 1 to 82 years. The results showed that 5-hmC content was significantly decreased and highly correlated with aging process, while 5-mC only showed slight correlation with age. We then established a DNA hydroxymethylation age model according to 5-hmC content with a mean absolute deviation (MAD) of approximate 8.9 years. We also calculated the mean relative error (MRE) using the predicted ages based on the age model and the chronological ages. The results showed that the MRE was 18.3 % for samples with ages from 20 to 82 years (95 % confidence interval, N = 190).

CONCLUSIONS: The global DNA hydroxymethylation represents a strong and reproducible mark of chronological age, which could be potentially applied in health assessment and prevention of diseases. The identification of biological or environmental factors that influence DNA hydroxymethylation aging rate may permit quantitative assessments of their impacts on health.

Keywords: 5-hydroxymethylcytosine; 5-methylcytosine; Aging; Hydrophilic-interaction liquid chromatography; Mass spectrometry

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