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Smith NJ, Hinley J, Varley CL, et al. The human urothelial tight junction: claudin 3 and the ZO-1α. Bladder (San Franc). 2015;2(1):e9doi: 10.14440/bladder.2015.33.
Smith, N. J., Hinley, J., Varley, C. L., Eardley, I., Trejdosiewicz, L. K., & Southgate, J. (2015). The human urothelial tight junction: claudin 3 and the ZO-1α. Bladder (San Francisco, Calif.), 2(1), e9. https://doi.org/10.14440/bladder.2015.33
Smith, Nicholas J, et al. "The human urothelial tight junction: claudin 3 and the ZO-1α." Bladder (San Francisco, Calif.) vol. 2,1 (2015): e9. doi: https://doi.org/10.14440/bladder.2015.33
Smith NJ, Hinley J, Varley CL, Eardley I, Trejdosiewicz LK, Southgate J. The human urothelial tight junction: claudin 3 and the ZO-1α. Bladder (San Franc). 2015;2(1):e9. doi: 10.14440/bladder.2015.33. PMID: 26269793; PMCID: PMC4530542.
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Bladder (San Franc). 2015;2(1):e9. doi: 10.14440/bladder.2015.33.

The human urothelial tight junction: claudin 3 and the ZO-1α.

Bladder (San Francisco, Calif.)

Nicholas J Smith, Jennifer Hinley, Claire L Varley, Ian Eardley, Ludwik K Trejdosiewicz, Jennifer Southgate

Affiliations

  1. Jack Birch Unit of Molecular Carcinogenesis, Department of Biology, University of York, York YO10 5DD, United Kingdom ; Pyrah Department of Urology, St James's University Hospital, Leeds LS9 7TF, United Kingdom.
  2. Jack Birch Unit of Molecular Carcinogenesis, Department of Biology, University of York, York YO10 5DD, United Kingdom.
  3. Pyrah Department of Urology, St James's University Hospital, Leeds LS9 7TF, United Kingdom.

PMID: 26269793 PMCID: PMC4530542 DOI: 10.14440/bladder.2015.33

Abstract

OBJECTIVE: Tight junctions are multicomponent structures, with claudin proteins defining paracellular permeability. Claudin 3 is a candidate for the exceptional "tightness" of human urothelium, being localised to the terminal tight junction (TJ) of superficial cells. Our aim was to determine whether claudin 3 plays an instigating and/or a functional role in the urothelial TJ.

MATERIALS AND METHODS: Normal human urothelial (NHU) cells maintained as non-immortalised cell lines were retrovirally-transduced to over-express or silence claudin 3 expression. Stable sublines induced to stratify or differentiate were assessed for TJ formation by immunocytochemistry and transepithelial electrical resistance (TER). Expression of claudin 3, ZO-1 and ZO-1α

RESULTS: Claudin 3 expression was associated with differentiation and development of a tight barrier and along with ZO-1 and ZO-1α

CONCLUSIONS: Urothelial cytodifferentiation is accompanied by induction of claudin 3 which is essential for the development of a terminal TJ. A coordinated switch to the ZO-1α

Keywords: claudin; differentiation; tight junction; urothelium; zonula occludens

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